Isolation and characterization of two novel root-specific promoters in rice (Oryza sativa L.)
Isolation and characterization of two novel root-specific promoters in rice (Oryza sativa L.)
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水稻 (Oryza sativa L.) 中两个新型根特异性启动子的分离和表征
DOI：
10.1016/j.plantsci.2013.02.002
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发表时间：
2013-06-01
期刊：
影响因子：
5.2
通讯作者：
Wu, Ping
中科院分区：
文献类型：
--
作者：
Li, Yuanya;Liu, Shaojun;Wu, Ping
新的根特异性启动子对于开发驱动根特异性基因表达以用于营养和水分吸收的方法是重要的。RT-PCR（反转录聚合酶链反应）分析鉴定了Os 03 g 01700和Os 02 g37190在不同发育阶段的根组织中的高表达水平，与组成型基因OsAct 1（水稻Actin 1基因）、OsUbi 1（水稻多聚泛素rubi 1基因）和OsCc 1（水稻细胞色素c基因）相比。通过qRT-PCR评估0 s 03 g 01700和0 s 02 g37190的拷贝数。结果表明，Os 03 g 01700和Os 02 g37190转录本在所检测的根组织中高度积累，但在幼胚或萌发后指定天数的叶片或穗中未检测到，与OsAct 1、OsUbi 1和OsCc 1的普遍表达相反。另外，将这两个基因的启动子区与GUS plus报告基因连接并转化到水稻中。GUS染色结果表明，Os 03 g 01700和Os 02 g37190启动子除了在根毛中表达外，在整个发育阶段的初生根和次生根中都有表达。转基因水稻中GUS Plus的转录水平也具有高度的根特异性。总体而言，这两个启动子在水稻根组织中具有高活性，并且可用于目的基因的根特异性增强。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Novel root-specific promoters are important for developing methods to drive root-specific gene expression for nutrient and water absorption. RT-PCR (reverse transcription polymerase chain reaction) analysis identified high expression levels of Os03g01700 and Os02g37190 in root tissues across developmental stages in comparison with the constitutive genes OsAct1 (rice Actin1 gene), OsUbi1 (rice polyubiquitin rubi1 gene), and OsCc1 (rice cytochrome c gene). The copy numbers of Os03g01700 and Os02g37190 were evaluated by qRT-PCR. The results showed that Os03g01700 and Os02g37190 transcripts were highly accumulated in the examined root tissues but were not detected in young embryos or leaves at the indicated days after germination or in the panicle, in contrast to the ubiquitous expression of OsAct1, OsUbi1, and OsCc1. Additionally, the promoter regions of these two genes were linked to the GUSplus reporter gene and transformed into rice. GUS staining of the transgenic plants showed that the Os03g01700 and Os02g37190 promoters were active in primary and secondary roots throughout the developmental stages, except in root hairs. The GUSPlus transcript levels were also highly root-specific in the transgenic rice. Overall, the two promoters are highly active in the root tissues of rice and can be useful for the root-specific enhancement of target gene(s). (C) 2013 Elsevier Ireland Ltd. All rights reserved.