Deletion of the ubiquitin-conjugating enzyme Ubc2 confers resistance to methylmercury in budding yeast by promoting Whi2 degradation.
Deletion of the ubiquitin-conjugating enzyme Ubc2 confers resistance to methylmercury in budding yeast by promoting Whi2 degradation.
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删除泛素结合酶 Ubc2 通过促进 Whi2 降解而赋予芽殖酵母对甲基汞的抗性。
DOI：
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发表时间：
2013
影响因子：
2
通讯作者：
A. Naganuma
中科院分区：
文献类型：
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作者：
Gi;Fujio Mastuyama;Tsutomu Takahashi;Jin;A. Naganuma
通过缺失分析确定了酵母中参与甲基汞敏感性的泛素结合酶，这表明Ubc 2-或Ubp 13-缺陷赋予对甲基汞的抗性。Whi 2，这是以前被证明是与甲基汞毒性增加，并通过泛素-蛋白酶体系统在细胞内降解，表达在显着较低的水平在Ubc 2缺陷型酵母比野生型酵母。Ubc 2/Whi 2双缺陷酵母既没有表现出添加剂，也没有协同增加甲基汞抗性。我们的研究结果表明，Ubc 2可能会增加酵母甲基汞的敏感性，通过抑制蛋白酶体降解Whi 2。
Ubiquitin-conjugating enzymes involved in sensitivity to methylmercury in yeast were identified by deletion analysis, which showed that Ubc2- or Ubp13-deficiency conferred resistance to methylmercury. Whi2, which was previously shown to be associated with increased methylmercury toxicity and is intracellularly degraded via the ubiquitin-proteasome system, was expressed at significantly lower levels in Ubc2-deficient yeast than in wild-type yeast. Ubc2/Whi2 double-deficient yeast showed neither an additive nor synergistic increase in methylmercury resistance. Our results indicate that Ubc2 may increase the sensitivity to methylmercury in yeast by inhibiting the proteasomal degradation of Whi2.