Recent developments in methacrylate embedding. I. A study of the polymerization damage phenomenon by phase contrast microscopy
Recent developments in methacrylate embedding. I. A study of the polymerization damage phenomenon by phase contrast microscopy
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DOI：
10.1083/jcb.2.4.3
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发表时间：
1956-01-01
期刊：
影响因子：
--
通讯作者：
BORYSKO, EMIL
中科院分区：
文献类型：
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作者：
BORYSKO, EMIL
将在组织培养中生长的活细胞的外观与包埋过程的每个阶段后相同细胞的外观进行比较。渗压固定后未观察到大体变化。在某些情况下，用乙醇脱水产生体积几乎为30%的球形细胞收缩。在包埋基质的聚合过程中产生了最严重的扭曲，表现为细胞肿胀。变形程度在不同制备物之间以及在同一制备物内的点与点之间变化。通过使用高聚合温度（60[degree]C和80[degree]C）使这种类型的变形最小化。有人建议，聚合损伤是由内部应力造成的嵌入质量内的聚合收缩率的不均匀性。通过使用高聚合温度，甲基丙烯酸酯保持在流体状态，通过流动释放内应力，并使对细胞的损害最小化。|| 摘要：E.博雷斯科
The appearance of living cells, grown in tissue culture, was compared with the appearance of the same cells after each phase of the embedding process. Gross changes were not observed after osmic fixation. Dehydration with ethanol produced a shrinkage of spherical cells of almost 30% in volume in some cases. The most severe distortions were produced during the polymerization of the embedding matrix, taking the form of a swelling of the cells. The degree of distortion varied from one preparation to another as well as from point to point within the same preparation.. This type of distortion was minimized by using high polymerization temperatures (60[degree]C and 80[degree]C). It is suggested that polymerization damage is caused by internal stresses resulting from non-uniformity in the rate of polymerization contraction within the embedding mass. By using high polymerization temperatures, the methacrylate is maintained in a fluid condition, internal stresses are relieved by flow and damage to the cells is minimized. || ABSTRACT AUTHORS: E. Borysko