RNA sequencing reveals the involvement of quorum sensing in dairy spoilage caused by psychrotrophic bacteria
RNA sequencing reveals the involvement of quorum sensing in dairy spoilage caused by psychrotrophic bacteria
复制标题
RNA测序揭示群体感应与耐冷细菌引起的乳制品腐败有关
DOI：
10.1016/j.lwt.2020.109384
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发表时间：
2020-06
影响因子：
6
通讯作者：
He Guoqing
中科院分区：
文献类型：
--
作者：
Yuan Lei;Wang Ni;Sadiq Faizan A.;He Guoqing
据报道，嗜冷菌引起的乳制品腐败受基于酰基-高丝氨酸-内酯(AHLS)的群体感应(QS)系统的调控。然而，嗜冷菌产生的AHL在乳制品变质中的确切作用尚不清楚。本研究的目的是在转录水平上评价AHLS对偶氮假单胞菌和液化葡萄球菌引起的乳制品腐败的影响。选择牛奶的颗粒大小和挥发性化合物作为变质指标，考察AHLS在乳制品变质中的作用。在此基础上，首次利用RNA测序技术对AHLS处理过的牛奶冷藏过程中嗜冷菌的基因表达进行了研究。结果表明，外源AHLS促进了乳粒大小和乳酸量的增加，证实了AHLS对牛奶品质劣变的促进作用。转录组分析表明，当外源AHLS处理时，菌株的许多核心基因都有明显的差异表达，QS协调了供试菌株的代谢过程。大多数涉及细菌生长代谢、酶的产生和胁迫反应的基因都受到外源AHLS的上调。这些结果阐明了AHL在嗜冷菌引起的乳制品腐败中的潜在作用。这将为控制乳制品中微生物腐败的新策略提供线索。
Dairy spoilage caused by psychrotrophic bacteria has been reported to be regulated by acyl-homoserine-lactones (AHLs)-based quorum sensing (QS) system. However, the precise role of AHLs produced by psychrotrophic bacteria in dairy spoilage remains unclear. The aim of this study was to evaluate the effects of AHLs on dairy spoilage caused by PseudomonasazotoformansandSerrita liquefaciensat the transcriptional level. The particle size and volatile compounds of milk were selected as spoilage indicators for investigating the involvement of AHLs in dairy spoilage. Furthermore, thein situgene expressions of psychrotrophic bacteria in AHLs-treated milk during cold storage were explored by RNA sequencing for the first time. The results revealed that the milk particle size and content of acids in milk were increased by exogenous AHLs, which confirm the enhancing effect of AHLs on the quality deterioration of milk. Transcriptome analysis revealed that a number of core genes of strains were significantly differentially expressed when treated with exogenous AHLs, and QS coordinate the metabolic processes within tested strains. Most of genes involving in the bacterial growth and metabolism, enzymes production, and stress response were up-regulated by exogenous AHLs. The results elucidate the potential involvement of AHLs in dairy spoilage caused by psychrotrophic bacteria. This would give clue for novel strategy of controlling microbial spoilage in dairy products.
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影响因子：
3.2
作者：
R. Van Houdt;P. Moons;Maria Hueso Buj;C. Michiels
通讯作者：
R. Van Houdt;P. Moons;Maria Hueso Buj;C. Michiels
影响因子：
3.2
作者：
Lindgren, J. K.;Thomas, V. C.;Fey, P. D.
通讯作者：
Fey, P. D.
影响因子：
4.4
作者：
Decimo, Marilu;Cabeza, Maria C.;Brasca, Milena
通讯作者：
Brasca, Milena
影响因子：
8.8
作者：
Valero, E;Villamiel, M;Martínez-Castro, I
通讯作者：
Martínez-Castro, I
影响因子：
3.5
作者：
M. Stuknytė;M. Decimo;M. Colzani;T. Silvetti;Milena Brasca;S. Cattaneo;G. Aldini;I. D. Noni
通讯作者：
M. Stuknytė;M. Decimo;M. Colzani;T. Silvetti;Milena Brasca;S. Cattaneo;G. Aldini;I. D. Noni