Structure of nonhairpin coding-end DNA breaks in cells undergoing V(D)J recombination
Structure of nonhairpin coding-end DNA breaks in cells undergoing V(D)J recombination
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DOI：
10.1128/mcb.18.4.2029
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发表时间：
1998-04-01
影响因子：
5.3
通讯作者：
Schlissel, MS
中科院分区：
文献类型：
--
作者：
Schlissel, MS
V（D）J重组酶识别两侧为重组信号序列的一对免疫球蛋白或T细胞受体基因片段，并引入双链断裂，产生两个信号末端和两个编码末端。断裂的编码末端最初被鉴定为共价闭合的发夹DNA分子。然而，在重组之前，发夹必须被打开，并且末端必须通过核酸酶消化和N-区添加来修饰。我们现在已经分析了非发夹编码结束与各种免疫球蛋白基因片段的细胞进行V（D）J重组。我们发现，这些断裂的DNA末端具有不同的非随机5 '链缺失，这是每一个的特征！这些缺失与涉及这些基因片段的编码接头的序列特征密切相关。此外，与断裂的信号末端不同，这些非发夹编码末端V（D）J重组反应中间体具有3'突出末端，我们讨论了这些结果的含义对于编码接头形成期间如何发生序列修饰的模型。
The V(D)J recombinase recognizes a pair of immunoglobulin or T-cell receptor gene segments flanked by recombination signal sequences and introduces double-strand breaks, generating two signal ends and two coding ends, Broken coding ends were initially identified as covalently closed hairpin DNA molecules. Before recombination, however, the hairpins must be opened and the ends must be modified by nuclease digestion and N-region addition. We have now analyzed nonhairpin coding ends associated with various immunoglobulin gene segments in cells undergoing V(D)J recombination. We found that these broken DNA ends have different nonrandom 5'-strand deletions which were characteristic for each! locus examined, These deletions correlate well with the sequence characteristics of coding joints involving these gene segments, In addition, unlike broken signal ends, these nonhairpin coding-end V(D)J recombination reaction intermediates have 3' overhanging ends, We discuss the implications of these results for models of how sequence modifications occur during coding-joint formation.