Thermotolerant Bacillus kokeshiiformis sp nov isolated from marine animal resources compost
Thermotolerant Bacillus kokeshiiformis sp nov isolated from marine animal resources compost
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DOI：
10.1099/ijs.0.059329-0
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发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Sakai, Kenji
中科院分区：
文献类型：
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作者：
Poudel, Pramod;Miyamoto, Hirokuni;Sakai, Kenji
从海洋动物资源（MAR）堆肥中分离到一株革兰氏染色阳性、内孢子形成、杆状、兼性厌氧、耐热的细菌，命名为MO-04（T）。MO-04（T）菌株的16 S rRNA基因序列与嗜热芽孢杆菌R-6488（T）、嗜热解淀粉芽孢杆菌CNCM I-1378（T）、腐殖芽孢杆菌LMG 22167（T）的同源性分别为99.4%、94.1%、93.3%、93.3%。与Bacillus niacini IFO 15566（T）的相似性为93.2%，与其它物种的相似性均小于93%。菌株MO-04（T）与菌株B的DNA亲缘关系。thermolactis DSM 23332（T）的产率为45%。菌株MO-04（T）的DNA G+C含量为33.4 mol%，低于菌株B。thermolactis R6488（T）（35.0摩尔%）。菌株MO-04（T）在35-61 ℃（最适50 ℃）、pH 4.5-9.0（最适pH 7.2）和耐受高达8.0%（w/v）NaCl（最适2%）下生长。MO-04（T）细胞壁肽聚糖类型为内消旋-2，6-二氨基庚二酸，主要脂肪酸为C-16：1、C-14：1、C-17：0和C-17：1。主要极性脂质由二磷脂酰甘油和磷脂酰甘油以及两种未鉴定的磷脂代表。本文提供的多相分析数据清楚地表明，分离物MO-04（T）被认为是芽孢杆菌属中的一个新种，并建议将其命名为芽孢杆菌kokeshiiformis sp. nov.。B的模式菌株。kokeshiformis是MO-04（T）（=JCM 19325（T）=KCTC 33163（T））。
A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04(T), was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04(T) showed 99.4% similarity with Bacillus thermolactis R-6488(T), 94.1% similarity with Bacillus thermoamylovorans CNCM I-1378(T), 93.3% similarity with Bacillus humi LMG 22167(T), 93.2% similarity with Bacillus niacini IFO 15566(T) and the similarities with other species were less than 93%. DNA DNA relatedness between strain MO-04(T) and B. thermolactis DSM 23332(T) was 45%. The DNA G+C content of strain MO-04(T) was 33.4 mol%, comparatively lower than that of B. thermolactis R6488(T) (35.0 mol%). Strain MO-04(T) grew at 35-61 degrees C (optimum 50 C), pH 4.5-9.0 (optimum pH 7.2) and tolerated up to 8.0 % (w/v) NaCl (optimum 2%). The MO-04(T) cell wall peptidoglycan type was meso-2,6-diaminopimelic acid, and the major fatty acids were C-16:1, C-14:1, C-17:0 and C-17:1. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04(T) is considered to represent a novel species within the genus Bacillus for which the name Bacillus kokeshiiformis sp. nov. is proposed. The type strain of B. kokeshiiformis is MO-04(T) (=JCM 19325(T)=KCTC 33163(T)).