Characteristics of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets with diarrhea in central and southern Taiwan in 2015.
Characteristics of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets with diarrhea in central and southern Taiwan in 2015.
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DOI：
10.1186/s12917-017-0986-7
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发表时间：
2017-03-01
影响因子：
2.6
通讯作者：
Yeh KS
中科院分区：
文献类型：
--
作者：
Lee WC;Yeh KS
超广谱β-内酰胺酶（ESBLs）的产生使其对常用的β-内酰胺类抗生素产生耐药性，并且产ESBLs的细菌在人和兽医学中造成治疗困难。近年来，在牲畜中出现了产ESBL细菌，这可能引起人们对此类细菌可能通过食物链转移的担忧。养猪业在台湾是重要的，但对猪的ESBL状况的调查有限。我们于2015年1月至12月在台湾中部和南部的16个养猪场收集了275份腹泻仔猪的粪便拭子样本，并对其进行了产ESBL大肠杆菌筛查。ESBL产生菌的表型和基因型分别用联合纸片法和聚合酶链反应及DNA测序法确定。产ESBL E.大肠杆菌感染率为19.7%（54/275），且全部来自台湾南部的养猪场。bla CTX-M-1-组和bla CTX-M-9-组是发现的两个bla CTX-M组。bla CTX-M-1-组的bla CTX-M-55（34/54; 63.0%）和bla CTX-M-15（16/54; 29.6%）是两个主要bla基因类型，而bla CTX-M-65是在bla CTX-M-9组中发现的唯一类型。27株含有bla TEM-1，另外27株含有bla TEM-116。在屏东发现的一个菌株携带三种bla基因：bla TEM-116、bla CTX-M-55和bla CTX-M-65。产ESBL E.多位点序列分型结果显示，ST 10克隆复合物（包括ST 10、167、44和617）占35%（19/54）。产ESBL E.大肠杆菌从腹泻仔猪分离位于台湾南部的养猪场。最常检测到的bla是bla CTX-M-15和bla CTX-M-55。ST 10克隆复合物包含了我们的大部分产ESBL的E.大肠杆菌菌株。猪只排出的粪便可能污染环境，导致公共卫生问题;因此，持续监测猪只和其他食用动物的ESBL是必不可少的。
The production of extended-spectrum β-lactamases (ESBLs) confer resistance to the commonly used beta-lactam antimicrobials and ESBL–producing bacteria render treatment difficulty in human and veterinary medicine. ESBL–producing bacteria have emerged in livestock in recent years, which may raise concerns regarding possible transfer of such bacteria through the food chain. The swine industry is important in Taiwan, but investigations regarding the status of ESBL in swine are limited. We collected 275 fecal swab samples from piglets with diarrhea in 16 swine farms located in central and southern Taiwan from January to December 2015 and screened them for ESBL–producing Escherichia coli. ESBL producers were confirmed phenotypically by combination disc test and genotypically by polymerase chain reaction and DNA sequencing. The occurrence rate of ESBL–producing E. coli was 19.7% (54 of 275), and all were obtained in swine farms located in southern Taiwan. bla CTX-M-1-group and bla CTX-M-9-group were the two bla CTX-M groups found. bla CTX-M-55 (34 of 54; 63.0%) and bla CTX-M-15 (16 of 54; 29.6%), which belong to the bla CTX-M-1-group, were the two major bla gene types, whereas bla CTX-M-65 was the only type found in the bla CTX-M-9 group. Twenty-seven strains contained bla TEM-1, and the other 27 strains contained bla TEM-116. One strain found in Pingtung harbored three bla genes: bla TEM-116, bla CTX-M-55, and bla CTX-M-65. ESBL–producing E. coli exhibited a multidrug-resistant phenotype, and multilocus sequence typing revealed that the ST10 clonal complexes, including ST10, 167, 44, and 617 accounted for 35% (19 of 54) of these strains. ESBL-producing E. coli from piglets with diarrhea were isolated from swine farms located in southern Taiwan. The most commonly detected bla were bla CTX-M-15 and bla CTX-M-55. The ST10 clonal complexes comprised most of our ESBL-producing E. coli strains. Fecal shedding from swine may contaminate the environment, resulting in public health concerns; thus, continued surveillance of ESBL is essential in swine and in other food animals.