Shared Genomic Regions Between Derivatives of a Large Segregating Population of Maize Identified Using Bulked Segregant Analysis Sequencing and Traditional Linkage Analysis.
Shared Genomic Regions Between Derivatives of a Large Segregating Population of Maize Identified Using Bulked Segregant Analysis Sequencing and Traditional Linkage Analysis.
复制标题
使用批量分离分析测序和传统连锁分析鉴定出大量玉米分离群体衍生物之间的共享基因组区域。
DOI：
10.1534/g3.115.017665
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发表时间：
2015-06-01
期刊：
影响因子：
--
通讯作者：
de Leon N
中科院分区：
文献类型：
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作者：
Haase NJ;Beissinger T;Hirsch CN;Vaillancourt B;Deshpande S;Barry K;Buell CR;Kaeppler SM;de Leon N
从营养生长阶段到生殖生长阶段的延迟过渡和植物高度的增加已被证明可以增加禾本科植物的生物量生产力。本项目的目标是检测数量性状基因座使用极端从一个大的合成人口，以及相关的重组自交系定位人口这两个性状。来自B73 × Mo17非近交群体（IBM Syn14）的10，000个个体在大约16，500株植物ha−1的密度下种植，该群体交配了14代。开花时间和植株高度在这个群体内进行了测量。将来自每个分布尾部的46个最极端个体的DNA合并用于测量的每个性状并用于批量分离分析（BSA）测序。在10110万个SNP位点中的每一个上的等位基因分歧被估计为所选择的极端之间的等位基因频率的差异。此外，将224个交配的B73 × Mo17重组自交系以相似的密度与大的合成群体相邻地伴随生长，并评估开花时间和植株高度。使用BSA测序方法，14和13个基因组区域分别确定了开花时间和株高。与RIL群体的连锁图谱确定了8个和3个地区的开花时间和株高，分别。确定的区域，三个共定位的两个种群之间的开花时间和两个共定位的株高。这项研究表明，使用BSA测序的复杂的数量性状的解剖重要的木质纤维素乙醇生产的效用。
Delayed transition from the vegetative stage to the reproductive stage of development and increased plant height have been shown to increase biomass productivity in grasses. The goal of this project was to detect quantitative trait loci using extremes from a large synthetic population, as well as a related recombinant inbred line mapping population for these two traits. Ten thousand individuals from a B73 × Mo17 noninbred population intermated for 14 generations (IBM Syn14) were grown at a density of approximately 16,500 plants ha−1. Flowering time and plant height were measured within this population. DNA was pooled from the 46 most extreme individuals from each distributional tail for each of the traits measured and used in bulk segregant analysis (BSA) sequencing. Allelic divergence at each of the ∼1.1 million SNP loci was estimated as the difference in allele frequencies between the selected extremes. Additionally, 224 intermated B73 × Mo17 recombinant inbred lines were concomitantly grown at a similar density adjacent to the large synthetic population and were assessed for flowering time and plant height. Using the BSA sequencing method, 14 and 13 genomic regions were identified for flowering time and plant height, respectively. Linkage mapping with the RIL population identified eight and three regions for flowering time and plant height, respectively. Of the regions identified, three colocalized between the two populations for flowering time and two colocalized for plant height. This study demonstrates the utility of using BSA sequencing for the dissection of complex quantitative traits important for production of lignocellulosic ethanol.