peg10, an imprinted gene, plays a crucial role in adipocyte differentiation
peg10, an imprinted gene, plays a crucial role in adipocyte differentiation
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DOI：
10.1016/j.febslet.2007.07.074
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发表时间：
2007-09-04
期刊：
影响因子：
3.5
通讯作者：
Imagawa, Masayoshi
中科院分区：
文献类型：
--
作者：
Hishida, Tomoaki;Naito, Kumiko;Imagawa, Masayoshi
一个印记基因，父系表达基因（peg）10，被分离为脂肪形成早期表达的基因之一。peg 10的表达在添加诱导剂后升高，并且在脂肪细胞可分化的3 T3-L1细胞中检测到，但在非脂肪细胞系NIH 3 T3中未观察到。此外，通过RNA干扰（RNAi）敲低peg 10抑制3 T3-L1细胞分化为充满脂质的脂肪细胞。有趣的是，peg 10 RNAi处理降低了C/EBP β和C/EBP δ的表达，并抑制了有丝分裂克隆扩增。这些发现有力地表明，peg 10在脂肪细胞分化的早期阶段起着至关重要的作用。(c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
An imprinted gene, paternally expressed gene (peg) 10, was isolated as one of the genes expressed early in adipogenesis. The expression of peg10 was elevated after the addition of inducers, and was detected in adipocyte differentiable 3T3-L1 cells, but not observed in the non-adipogenic cell line NIH3T3. Moreover, the knockdown of peg10 by RNA interference (RNAi) inhibited the differentiation of 3T3-L1 cells into lipid-laden adipocytes. Interestingly, peg10 RNAi-treatment reduced the expressions of C/EBP beta and C/EBP delta, and inhibited mitotic clonal expansion. These findings strongly indicate that peg10 plays a crucial role at the immediate early stage of adipocyte differentiation. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.