LOCATION OF PHOSPHORYLATION SITE AND DNA-BINDING SITE OF A POSITIVE REGULATOR, OMPR, INVOLVED IN ACTIVATION OF THE OSMOREGULATORY GENES OF ESCHERICHIA-COLI
LOCATION OF PHOSPHORYLATION SITE AND DNA-BINDING SITE OF A POSITIVE REGULATOR, OMPR, INVOLVED IN ACTIVATION OF THE OSMOREGULATORY GENES OF ESCHERICHIA-COLI
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DOI：
10.1016/0014-5793(89)80617-9
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发表时间：
1989-06-05
期刊：
影响因子：
3.5
通讯作者：
MIZUNO, T
中科院分区：
文献类型：
--
作者：
KATO, M;AIBA, H;MIZUNO, T
大肠杆菌的OmpR蛋白是一种参与ompF和ompC基因激活的正调控因子，ompF和ompC基因分别编码主要的外膜蛋白OmpF和OmpC。通过采用重组DNA技术，我们分离了OmpR分子的N-和C-末端的一半。根据这些片段的生化分析结果，可以得出结论，N末端部分包含参与OmpR特异性蛋白激酶EnvZ磷酸化的位点，而C末端部分具有ompCandompF启动子的DNA结合位点。
The OmpR protein ofEscherichia coliis a positive regulator involved in activation of theompFandompCgenes which encode the major outer membrane proteins OmpF and OmpC, respectively. By employing recombinant DNA techniques, we isolated the N‐ and C‐terminal halves of the OmpR molecule. From the results of biochemical analyses of these fragments, it was concluded that the N‐terminal portion contains a site involved in phosphorylation by an OmpR‐specific protein kinase EnvZ, whereas the C‐terminal part possesses a DNA‐binding site for theompCandompFpromoters.