FtsZ-spirals and -arcs determine the shape of the invaginating septa in some mutants of Escherichia coli
FtsZ-spirals and -arcs determine the shape of the invaginating septa in some mutants of Escherichia coli
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DOI：
10.1046/j.1365-2958.1996.00100.x
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发表时间：
1996-10-01
影响因子：
3.6
通讯作者：
Lutkenhaus, J
中科院分区：
文献类型：
--
作者：
Addinall, SG;Lutkenhaus, J
细胞分裂必需蛋白FtsZ在未来的分裂位点形成动态的环状结构。这个Z环在细胞分裂期间收缩，同时保持在内陷隔膜的前缘处的位置。使用免疫荧光显微镜，我们的特点是两种情况下，形成非环FtsZ结构。在ftsZ 26（温度敏感，Ts）突变细胞中，形成FtsZ-螺旋并导致内陷隔膜的形成，这反过来导致形态异常。在rodA（sui）突变细胞，生长为球体，而不是杆，FtsZ-弧形成的不对称中隔内陷开始。FtsZ弧后来成熟为完整的FtsZ环。我们的数据表明，Z-螺旋和Z-弧可以收缩，并且在这样做的过程中，它们决定了内陷隔膜的形状。这些结果也强烈表明，在正常的细胞分裂，FtsZ定位到内膜上的单个成核位点，从那里它围绕细胞圆周双向聚合形成Z环。
The essential cell division protein FtsZ forms a dynamic ring structure at the future division site. This Z-ring contracts during cell division while maintaining a position at the leading edge of the invaginating septum. Using immunofluorescence microscopy we have characterized two situations in which non-ring FtsZ structures are formed. In ftsZ26 (temperature sensitive, Ts) mutant cells, FtsZ-spirals are formed and lead to formation of spirally invaginating septa, which in turn cause morphological abnormalities. In rodA(sui) mutant cells, which grow as spheres instead of rods, FtsZ-arcs are formed where asymmetric septal invaginations are initiated. The FtsZ-arcs later mature into complete FtsZ-rings. Our data show that Z-spirals and Z-arcs can contract and that in doing so, they determine the shape of the invaginating septa. These results also strongly suggest that in normal cell division, FtsZ is positioned to a single nucleation site on the inner membrane, from which it polymerizes bidirectionally around the cell circumference to form the Z-ring.