Differences in inhibition of the growth of commensal and enteropathogenic strains of Escherichia coli by lactotransferrin and secretory immunoglobulin A isolated from human milk.
Differences in inhibition of the growth of commensal and enteropathogenic strains of Escherichia coli by lactotransferrin and secretory immunoglobulin A isolated from human milk.
复制标题
DOI：
--
复制
发表时间：
1980-11
期刊：
影响因子：
6.4
通讯作者：
S. Stephens;J. Dolby;J. Montreuil;G. Spik
中科院分区：
文献类型：
--
作者：
S. Stephens;J. Dolby;J. Montreuil;G. Spik
研究了牛、人初乳和乳中的免疫球蛋白以及人乳中的乳转铁蛋白（LTF）对组织培养基中生长的大肠杆菌的抑菌活性。当单独测试时，从混合的人乳中提取的LTF或分泌性免疫球蛋白A （sIgA）对人类共生或肠致病性大肠杆菌菌株只有轻微的抑菌活性。总之，它们具有相当大的抑菌作用，但仅针对肠致病性血清型菌株。从人乳中分离出的sIgA对所有肠致病性血清型具有一致的活性，但从个别牛奶样品中分离出的sIgA对某些血清型无活性，这种特异性与针对O抗原的抗体有关。sIgA的活性在56度加热2小时时是稳定的，但在65度加热10分钟或更长时间时逐渐丧失。牛初乳IgGl单独无抑菌作用。与LTF一起，它对小牛致病性菌株有活性，但对人类肠致病性菌株没有活性。兔抗大肠杆菌共生致病性菌株血清的抑菌活性（与LTF相关）较高，且对诱导菌株的血清型具有特异性，但抗共生菌株血清的抑菌活性较低或不存在抑菌活性，尽管对这些菌株存在高滴度的凝集抗体。
Immunoglobulins from bovine and human colostrum and milk and lactotransferrin (LTF) from human milk were investigated for bacteriostatic activity against Escherichia coli growing in a tissue culture medium. When tested separately, LTF or secretory immunoglobulin A (sIgA) from pooled human milk showed only slight bacteriostatic activity against human commensal or enteropathogenic strains of E. coli. Together, they had a considerable bacteriostatic effect, but only against strains of enteropathogenic serotype. This activity of the sIgA from pooled human milk was consistent for all enteropathogenic serotypes tested, but sIgA isolated from individual milk samples was inactive against some serotypes, and this specificity was associated with antibody to the O antigens. The activity of the sIgA was stable to heat at 56 degrees for 2 h but was lost progressively on heating at 65 degrees for 10 min or longer. Bovine colostral IgGl was without bacteriostatic effect alone. Together with LTF, it was active against a strain pathogenic to calves but not against human enteropathogenic strains. Tests on rabbit antisera raised against commensal enteropathogenic strains of E. coli showed that for the enteropathogens the bacteriostatic activity (in association with LTF) was high and was specific for the serotype of the eliciting strain, but bacteriostatic activity was low or absent in the antisera to commensal strains in spite of the presence of high titres of agglutinating antibodies to these strains.