Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors
Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors
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DOI：
10.1126/science.1172408
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发表时间：
2009-05-22
期刊：
影响因子：
56.9
通讯作者：
Grill, Erwin
中科院分区：
文献类型：
--
作者：
Ma, Yue;Szostkiewicz, Izabela;Grill, Erwin
植物激素脱落酸（阿坝）作为一种发育信号，并作为一个集成器的环境线索，如干旱和寒冷。阿坝信号转导的关键参与者包括2C型蛋白磷酸酶（PP2Cs）ABI1和ABI2，它们通过负调节阿坝反应起作用。在这项研究中，我们确定了ABI1和ABI2的相互作用，我们将其命名为阿坝受体（RCAR）的调节成分。在拟南芥中，RCAR属于一个具有14个成员的家族，与第10类病原体相关蛋白具有结构相似性。RCAR 1被证明结合阿坝，介导ABA依赖性的体外ABI1或ABI2失活，并拮抗PP2C在植物中的作用。其他RCAR也介导ABI1和ABI2的ABA依赖性调节，与受体复合物的组合组装一致。
The plant hormone abscisic acid (ABA) acts as a developmental signal and as an integrator of environmental cues such as drought and cold. Key players in ABA signal transduction include the type 2C protein phosphatases (PP2Cs) ABI1 and ABI2, which act by negatively regulating ABA responses. In this study, we identify interactors of ABI1 and ABI2, which we have named regulatory components of ABA receptor (RCARs). In Arabidopsis, RCARs belong to a family with 14 members that share structural similarity with class 10 pathogen-related proteins. RCAR1 was shown to bind ABA, to mediate ABA-dependent inactivation of ABI1 or ABI2 in vitro, and to antagonize PP2C action in planta. Other RCARs also mediated ABA-dependent regulation of ABI1 and ABI2, consistent with a combinatorial assembly of receptor complexes.