Analysis of chromosome conservation in Lemur catta studied by chromosome paints and BAC/PAC probes
Analysis of chromosome conservation in Lemur catta studied by chromosome paints and BAC/PAC probes
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DOI：
10.1007/s00412-002-0215-3
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发表时间：
2002-12-01
期刊：
影响因子：
1.6
通讯作者：
Archidiacono, N
中科院分区：
文献类型：
--
作者：
Cardone, MF;Ventura, M;Archidiacono, N
利用一组人类染色体染色探针和细菌和PI人工染色体（BAC/PAC）克隆进行荧光原位杂交（FISH）实验，研究环尾狐猴（lemur catta， LCA）染色体相对于人类的保守性。人类染色体7、9、11、13、14、17、18、20、21和X特异的全染色体涂料被发现可以识别LCA中的单个染色体或不间断的染色体区域。然后使用大量的部分染色体涂料和BAC/PAC探针来完善区分两种核型的重排特征。结果也被用来重建祖先狐猴科的核型。狐猴的头壳确实可以作为一个外群，使得狐猴的同形（祖先）重排与同形（衍生）重排区别开来。一些LCA染色体在形态学上难以区分。大多数LCA染色体在特定探针上的“锚定”将有助于该物种核型的标准化。
A panel of human chromosome painting probes and bacterial and PI artificial chromosome (BAC/PAC) clones were used in fluorescence in situ hybridization (FISH) experiments to investigate the chromosome conservation of the ring-tailed lemur (Lemur catta, LCA) with respect to human. Whole chromosome paints specific for human chromosomes 7, 9, 11, 13, 14, 17, 18, 20, 21, and X were found to identify a single chromosome or an uninterrupted chromosomal region in LCA. A large set of partial chromosome paints and BAC/PAC probes were then used to refine the characterization of the rearrangements differentiating the two karyotypes. The results were also used to reconstruct the ancestral Lemuridae karyotype. Lemur catta, indeed, can be used as an outgroup, allowing symplesiomorphic (ancestral) rearrangements to be distinguished from apomorphic (derived) rearrangements in lemurs. Some LCA chromosomes are difficult to distinguish morphologically. The 'anchorage' of most LCA chromosomes to specific probes will contribute to the standardization of the karyotype of this species.