Morphological characterization of cardiomyocytes isolated from a transplantable cardiac tumor derived from transgenic mouse atria (AT-1 cells).
Morphological characterization of cardiomyocytes isolated from a transplantable cardiac tumor derived from transgenic mouse atria (AT-1 cells).
复制标题
从转基因小鼠心房（AT-1 细胞）衍生的可移植心脏肿瘤中分离出的心肌细胞的形态学特征。
DOI：
10.1161/01.res.69.6.1591
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发表时间：
1991
影响因子：
20.1
通讯作者：
Claycomb,WC
中科院分区：
文献类型：
--
作者：
Delcarpio,JB;LansonJr,NA;Field,LJ;Claycomb,WC
我们已经从表达SV40大T癌基因的转基因小鼠心房心肌细胞中开发出可移植的肿瘤谱系，并将这些心肌细胞命名为AT-1细胞。本研究采用相衬显微镜、放射自显影和电子显微镜对可移植肿瘤、新鲜分离的肿瘤心肌细胞和培养的肿瘤心肌细胞进行了检测。绝大多数皮下肿瘤细胞（占肿瘤细胞团的90%以上）表现为肉瘤带状。在原位和新分离的AT-1细胞中观察到体内心房心肌细胞的典型超微结构特征，包括组织良好的肌原纤维、间隙连接和心房特异性细胞质颗粒。那些不含有组织肌原纤维的细胞主要是血管成分，如内皮细胞。用[3H]胸苷标记表明，培养的AT-1细胞中有90%以上的细胞能够合成DNA；此外，可以看到许多细胞正在进行细胞分裂。电镜显示培养的AT-1心肌细胞具有上述所有特征，包括发育良好的横管系统。
We have developed a transplantable tumor lineage derived from transgenic mouse atrial cardiomyocytes that express the SV40 large T oncogene and have named these cardiomyocytes AT-1 cells. In this study, the transplantable tumors, freshly isolated tumor cardiomyocytes, and cultured tumor cardiomyocytes were examined using phase-contrast microscopy, autoradiography, and electron microscopy. The vast majority of the subcutaneous tumor cells, greater than 90% of the cellular mass of the tumor, exhibited sarcomeric banding. Ultrastructural characteristics typical of in vivo atrial cardiac muscle cells, including well-organized myofibrils, gap junctions, and atrial-specific cytoplasmic granules, were observed in in situ and in freshly isolated AT-1 cells. Those cells that did not contain some form of organized myofibrils were primarily vascular elements, such as endothelial cells. Labeling with [3H]thymidine indicated that greater than 90% of cultured AT-1 cells were synthesizing DNA; furthermore, many cells could be seen undergoing cell division. Electron microscopy revealed that the cultured AT-1 cardiomyocytes contained all of the above-described characteristics, including a well-developed transverse tubular system.