IDENTIFICATION OF 2 FORMS OF AN ENDOGENOUS MURINE RETROVIRAL ENV GENE LINKED TO THE RMCF LOCUS
IDENTIFICATION OF 2 FORMS OF AN ENDOGENOUS MURINE RETROVIRAL ENV GENE LINKED TO THE RMCF LOCUS
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DOI：
10.1128/jvi.61.1.29-34.1987
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发表时间：
1987-01-01
影响因子：
5.4
通讯作者：
PORTIS, JL
中科院分区：
文献类型：
--
作者：
BULLER, RS;AHMED, A;PORTIS, JL
Rmcf基因限制重组鼠貂细胞灶诱导（MCF）病毒在来自携带耐药等位基因（Rmcfr）小鼠的细胞培养物中的复制，并可能在体内逆转录病毒诱导的白血病耐药中发挥作用。我们的特点是内源性gp 70表达的Rmcfr和Rmcfs小鼠与一组类型特异性单克隆抗体，区分异嗜性和MCF gp 70。来自Rmcfr小鼠（DBA/2和CBA/N）的胚胎和尾部皮肤培养物表达带有MCF病毒特有决定子的gp 70，而来自Rmcfs小鼠的培养物表达不可检测的gp 70（NFS/N和IRW）或与异嗜性病毒亚组（C57 BL/6、CBA/J和A/WySn）血清学相关的gp 70。对（C57 BL/6 X DBA/2）X C57 BL/6回交后代胚胎的研究表明，Rmcf抗性等位基因与MCF gp 70的表达相关，C57 BL/6 Rmcfs小鼠表达的编码异嗜性gp 70的基因与Rmcfr小鼠的MCF gp 70等位。这些数据表明，Rmcf基因座含有内源性gp 70基因，具有两种等位基因形式，其中之一抑制外源性MCF感染在体外的病毒干扰机制。
The Rmcf gene restricts the replication of recombinant murine mink cell focus-inducing (MCF) viruses in cell cultures derived from mice carrying the resistance allele (Rmcfr) and may play a role in resistance to retrovirus-induced leukemias in vivo. We have characterized the endogenous gp70 expressed by Rmcfr and Rmcfs mice with a panel of type-specific monoclonal antibodies which discriminate xenotropic and MCF gp70. Embryo and tail skin cultures derived from Rmcfr mice (DBA/2 and CBA/N) expressed gp70 bearing a determinant unique to MCF viruses, whereas cultures from Rmcfs mice expressed either no detectable gp70 (NFS/N and IRW) or a gp70 serologically related to a subgroup of xenotropic viruses (C57BL/6, CBA/J, and A/WySn). Studies of progeny embryos derived from a (C57BL/6 X DBA/2) X C57BL/6 backcross established that the Rmcf resistance allele was linked to the expression of the MCF gp70 and that the gene encoding the xenotropic gp70 expressed by C57BL/6 Rmcfs mice was allelic with the MCF gp70 from Rmcfr mice. These data indicate that the Rmcf locus contains an endogenous gp70 gene having two allelic forms, one of which inhibits exogenous MCF infection in vitro by a mechanism of viral interference.