Prostaglandin synthesis by cells comprising the calf pulmonary artery
Prostaglandin synthesis by cells comprising the calf pulmonary artery
复制标题
由小牛肺动脉细胞合成前列腺素
DOI：
10.1002/jcp.1041200209
复制
发表时间：
1984
影响因子：
5.6
通讯作者：
P. Polgár
中科院分区：
文献类型：
--
作者：
M. Menconi;G. Hahn;P. Polgár
在包含牛肺动脉的三种细胞类型（内皮细胞、平滑肌细胞和成纤维细胞）中评价前列腺素（PG）的产生。前列环素（PG12）是内皮细胞、平滑肌细胞和成纤维细胞在培养或新鲜切除的组织碎片中产生的主要前列腺素（PG）。除PG1 2外，这些细胞还产生可测量量的PGE 2、PGF 2a和血栓烷A2（TXA2）。内皮细胞是PGs最活跃的生产者。然而，产生的PG的类型是特定细胞类型的特征，而生产水平取决于外部因素。前列腺素生产培养的细胞，无论是在基础条件下，并在刺激剂的反应，是非常相似的各自的新鲜切除的组织片段含有给定的细胞类型。培养物中的这些细胞可以被血管紧张素和缓激肽以非常低的（生理）浓度刺激产生PG1 2，这进一步表明培养物中这些细胞的生理反应性的保留。血管内皮细胞和成纤维细胞被浓度低至10−12 M的缓激肽激活，但对血管紧张素没有反应。原代和第一代培养物中的平滑肌细胞被10− 12 M浓度的缓激肽和血管紧张素激活。平滑肌细胞的连续传代培养导致其对缓激肽刺激的反应性逐渐丧失。细胞生长状态被证明是PG生产的重要决定因素。活跃生长的细胞在培养合成较少的PG相比，细胞已进入一个“静止”的非生长状态。
Prostaglandin (PG) production was evaluated in the three cell types (endothelial, smooth muscle, and fibroblast) comprising the bovine pulmonary artery. Prostacyclin (PG12) was the predominant prostaglandin (PG) produced by endothelial, smooth muscle, and fibroblast cells as they exist in culture or in freshly excised tissue fragments. In addition to PGl2, measurable amounts of PGE2, PGF2a, and thromboxane A2 (TXA2) were also produced by these cells. Endothelial cells were the most active producers of PGs. However, the type of PG produced was characteristic of the particular cell type, while the level of production was dependent on external factors. Prostaglandin production by cultured cells, both under basal conditions and in response to stimulatory agents, was quite similar to that of the respective freshly excised tissue fragments containing a given cell type. These cells in culture could be stimulated to produce PGl2 by both angiotensin and bradykinin at very low (physiological) concentrations, a further indication of the retention of the physiological responsiveness of these cells in culture. Endothelial cells and fibroblasts were activated by bradykinin at concentrations as low as 10−12 M but did not respond to angiotensin. Smooth muscle cells in primary and first passage cultures were activated by both bradykinin and angiotensin at 10−12M concentrations. Serial subcultivations of smooth muscle cells resulted in a progressive loss in their responsiveness to bradykinin stimulation. The state of cell growth proved to be an important determinant of PG production. Actively growing cells in culture synthesized less PG when compared to cells which had entered into a “quiescent” nongrowth state.
细胞生长和前列腺素合成的调节。
DOI：
10.1016/0090-6980(81)90211-2
发表时间：
1981
期刊：
Prostaglandins
影响因子：
--
作者：
Taylor,L;Polgar,P
通讯作者：
Polgar,P