Memantine protects neurons from shrinkage in the lateral geniculate nucleus in experimental glaucoma
Memantine protects neurons from shrinkage in the lateral geniculate nucleus in experimental glaucoma
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DOI：
10.1001/archopht.124.2.217
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发表时间：
2006-02-01
影响因子：
--
通讯作者：
Weinreb, RN
中科院分区：
文献类型：
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作者：
Yücel, YH;Gupta, N;Weinreb, RN
目的：确定美金刚治疗青光眼是否可以防止外侧膝状核（视网膜神经节细胞的主要靶标）的神经元萎缩。方法：对 16 只患有右眼单侧实验性青光眼的猴子进行了为期 14 个月的研究，并用美金刚 (n=9) 或仅用载体 (n=7) 进行治疗。小清蛋白免疫标记后检查左侧膝状核中继神经元（第 1、4 和 6 层）。使用无偏方法评估细胞体横截面积和神经元数量。使用 t 检验和协方差分析比较美金刚和载体治疗的青光眼组。 结果：与载体治疗的动物相比，美金刚治疗的动物在第 1 层（-4.0% +/- 13.9% vs 28.2% +/- 17.4%；P=.001）和第 4 层（24.9% +/- 10.0% vs 37.2%+/-12.3%； P=.04)。对于第 6 层，差异不具有统计显着性（34.2% +/- 10.1% vs 45.3% +/- 14.5%；P=.10）。协方差结果分析显示，美金刚治疗组第 1、4 和 6 层的神经元收缩显着减少（P
Objective: To determine whether memantine as a treatment for glaucoma prevents neuron shrinkage in the lateral geniculate nucleus, the major target for retinal ganglion cells.Methods: Sixteen monkeys with right-eye unilateral experimental glaucoma for 14 months were studied and treated with memantine (n=9) or vehicle only (n=7). Left lateral geniculate nucleus relay neurons (layers 1, 4, and 6) were examined following parvalbumin immunolabeling. Cell body cross-sectional areas and neuron numbers were assessed using unbiased methods. Memantine- and vehicle-treated glaucoma groups were compared using t tests and analysis of covariance.Results: Compared with vehicle-treated animals, memantine-treated animals showed significantly less mean +/- SD neuron shrinkage in layers 1 (-4.0% +/- 13.9% vs 28.2% +/- 17.4%; P=.001) and 4 (24.9% +/- 10.0% vs 37.2% +/- 12.3%; P=.04). For layer 6, the difference was not statistically significant (34.2% +/- 10.1% vs 45.3% +/- 14.5%; P=.10). Analysis of covariance results showed significantly less neuron shrinkage in the memantine-treated group for layers 1, 4, and 6 (P