Biomolecular study of the human remains from tomb 5859 in the Etruscan necropolis of Monterozzi, Tarquinia (Viterbo, Italy)
Biomolecular study of the human remains from tomb 5859 in the Etruscan necropolis of Monterozzi, Tarquinia (Viterbo, Italy)
复制标题
DOI：
10.1016/j.jas.2003.10.012
复制
发表时间：
2004-05-01
影响因子：
2.8
通讯作者：
Caramelli, D
中科院分区：
文献类型：
--
作者：
Cappellini, E;Chiarelli, B;Caramelli, D
在塔奎尼亚的蒙特罗齐墓地，考古发掘了伊特鲁里亚人的房间坟墓，发现了四个人。据推测，他们可能是一个家庭群体的成员。由于考古数据和经典人类学分析在这方面提供的信息很少，因此从个体的骨骼和牙齿碎片中提取了古代DNA （aDNA）。对每个受试者的线粒体DNA （mtDNA）的HVR-I进行克隆和测序。为了确定个体的性别，分析了amyelogenine和SRY基因。短串联重复序列（STR）也进行了鉴定。DNA研究之前进行氨基酸消旋程度评估和热重分析（TGA），以评估。分别为样品中有机物的降解和保存量。结果显示，两名受试者为男性，两名受试者为女性。此外，他们中的三个人有相同的mtDNA序列，因此，他们可能有母系关系。这一证据支持了一个假设，即坟墓的居住者可以被认为是一个由父母和他们的儿子和女儿组成的家庭群体的成员。分子研究提供了新的数据，以更好地定义以前提出的重建，仅基于形态学和考古学的方法。多学科调查也允许比较不同的方法和整合他们的贡献。(C) 2003 Elsevier Ltd.版权所有。
Archaeological excavation in an Etruscan room tomb, from the Monterozzi necropolis in Tarquinia led to the recovery of four individuals. It was hypothesized that they could be members of a single family group. As both archaeological data and classical anthropological analysis provided little information in this direction, ancient DNA (aDNA) was extracted from bone and tooth fragments of the individuals. For each subject HVR-I of the mitochondrial DNA (mtDNA) was cloned and sequenced. To identify the sex of the individuals, amelogenine and SRY genes were analysed. Short tandem repeat (STR) characterization was also performed. DNA studies were preceded by the evaluation of amino acids racemization extent and thermogravimetric analysis (TGA), to evaluate. respectively, degradation and quantity of organic matter preserved in the samples.Results show that two subjects are males, whereas two are females. Furthermore, three of them share the same mtDNA sequence, and, as such, they could be related by maternal lineage. This evidence supports the hypothesis that the occupants of the tomb can be considered members of a family group composing two parents and their son and daughter. Molecular study supplies new data to better define the reconstruction previously proposed, based only on a morphological and archaeological approach. Multidisciplinary investigation also allows comparison of the different method's and integration of their contributions. (C) 2003 Elsevier Ltd. All rights reserved.