Simultaneous estimation of Nadifloxacin and Mometasone Furoatein topical cream by HPTLC method
Simultaneous estimation of Nadifloxacin and Mometasone Furoatein topical cream by HPTLC method
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HPTLC法同时测定那氟沙星和糠酸莫米松外用乳膏的含量
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Poonam N. Ranjane
中科院分区：
文献类型：
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作者：
Amol A. Kulkarni;N. RabindraK.;Meenal N Ranjane;Poonam N. Ranjane
建立了用高效液相色谱法分析外用乳膏中纳氟沙星和糠酸莫米松含量的方法。以二氯乙烷：乙醚：氨：甲醇：乙酸乙酯（6:3:0.2:1.75:3.5 v/v）为流动相，在默克公司60f254预涂硅胶铝板上进行分离。密度扫描在254 nm处进行。纳地沙星浓度在1000 ~ 3000 ng/波段范围内呈线性，相关系数（r2 = 0.997）；糠酸莫米松浓度在100 ~ 300 ng/波段范围内呈线性，相关系数（r2 = 0.995）。该方法按照ICH指南进行了验证。该方法成功地应用于制剂中药物的分析。
The present work describes HPTLC method for analysis of Nadifloxacin and Mometasone furoate in topical cream. The separation was carried out on Merck precoated silica gel aluminium plate 60 F254 using Dichloroethane: Diethyl ether: Ammonia: Methanol: Ethyl acetate (6: 3: 0.2: 1.75: 3.5 v/v) as mobile phase. The densitometric scanning was carried out at 254 nm. Response was found to be linear in the concentration range of 1000–3000 ng /band with correlation coefficient (r2 = 0.997) for Nadifloxacin and 100-300 ng/band with correlation coefficient (r2 = 0.995) for Mometasone furoate. The method was validated as per ICH guidelines. The method was successfully applied for the analysis of drugs in pharmaceutical formulation.