The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina
The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina
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DOI：
10.1016/s0169-328x(00)00060-7
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发表时间：
2000-05-31
期刊：
影响因子：
--
通讯作者：
Barnstable, CJ
中科院分区：
文献类型：
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作者：
Baas, D;Bumsted, KM;Barnstable, CJ
最近的证据表明，含有同源结构域的蛋白质在中枢神经系统细胞命运的规范。在这里，我们报告说，在胚胎小鼠眼Otx2，成对的同源结构域转录因子，被发现在视网膜色素上皮细胞和视网膜神经元，包括神经节细胞的一个有限的子集。然而，在出生后和成人的眼睛，细胞和亚细胞分布的Otx 2蛋白的细胞类型特异性。Otx 2仅在视网膜色素上皮细胞和双极细胞的细胞核中表达，而在视杆细胞的细胞质中表达。视网膜外植体和转染细胞系的免疫组织化学研究都表明，在未成熟的杆细胞质中的Otx 2的保留是一个发育调节的过程。Otx 2在视杆细胞质和其他细胞类型的细胞核中的差异分布表明，该转录因子的亚细胞定位可能参与视网膜发育特定阶段的细胞命运决定。(C)2000 Elsevier Science B.V.保留所有权利。
Recent evidence implicates homeodomain-containing proteins in the specification of cell fates in the central nervous system. Here we report that in the embryonic mouse eye Otx2, a paired homeodomain transcription factor, was found in retinal pigment epithelial cells and a restricted subset of retinal neurons, including ganglion cells. In the postnatal and adult eye, however, both the cellular and subcellular distribution of the Otx2 protein were cell type-specific. Otx2 was detected only in the nuclei of retinal pigment epithelial and bipolar cells, but was present in the cytoplasm of rod photoreceptors. Immunohistochemical studies of retinal explants and transfected cell lines both suggested that the retention of Otx2 in the cytoplasm of immature rods is a developmentally regulated process. The differential distribution of Otx2 in the cytoplasm of rods and the nucleus of other cell types, suggests that subcellular localization of this transcription factor may participate cell fate determination during specific phases of retinal development. (C) 2000 Elsevier Science B.V. All rights reserved.