Monitoring Population Membrane Potential Signals from Neocortex
Monitoring Population Membrane Potential Signals from Neocortex
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DOI：
10.1007/978-1-4419-6558-5_7
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Wu, Jian-Young
中科院分区：
文献类型：
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作者：
Huang, Xiaoying;Xu, Weifeng;Wu, Jian-Young
电压敏感染料(VSD)和光学成像是研究大脑皮质组织中群体神经元活动的时空模式的有用工具。使用光电二极管阵列和吸收染料，我们能够同时从数百个点进行记录，其灵敏度可与局部场电位记录相媲美。由于吸收染料的光毒性比荧光染料小，因此每一片的总记录时间可达1,000-2,000 S，可分为数百次几个小时的短记录试验。在本章中，我们将讨论实现高灵敏度的可靠VSD成像的方法细节。
Voltage-sensitive dyes (VSDs) and optical imaging are useful tools for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes, we are able to record simultaneously from hundreds of points with sensitivity comparable to local field potential recordings. Since absorption dyes have less phototoxicity than fluorescent dyes, the total recording time in each slice can be 1,000–2,000 s, which can be divided into hundreds of short recording trials over several hours. In this chapter, we will discuss methodological details for achieving reliable VSD imaging with high sensitivity.