Expression of the basal cell adhesion molecule (B-CAM) in normal and diseased human skin
Expression of the basal cell adhesion molecule (B-CAM) in normal and diseased human skin
复制标题
DOI：
10.1034/j.1600-0560.2000.027003108.x
复制
发表时间：
2000-03-01
影响因子：
1.7
通讯作者：
Boehncke, WH
中科院分区：
文献类型：
--
作者：
Bernemann, TM;Podda, M;Boehncke, WH
基底细胞粘附分子（B-CAM）是一种90 kD的细胞表面糖蛋白，具有特征性的免疫球蛋白结构域结构。培养细胞中B-CAM表达的模式表明，该分子与某些谱系中的基质粘附生长模式相关。我们采用单批高滴度小鼠单克隆抗体G253，通过免疫组织化学方法研究了B-CAM在正常和患病人表皮中的表达。来自正常皮肤（n=8）、银屑病（n=5）、接触性皮炎（n=6）的速冻活检材料。基底细胞癌（n=5）和胎儿皮肤（n=6）进行了研究。在正常人的皮肤上。B-CAM在整个表皮中不同程度地被发现，具有基底上表达的偏好，毛囊有规律地具有B-CAM阳性表型。正常皮肤病与银屑病和接触性皮炎相比，B-CAM表达模式没有质的差异。与此相反，胎儿皮肤（妊娠第15至18周）的特点是B-CAM阳性细胞在基底层的表皮，以及在外根鞘的毛囊。基底细胞癌也经常表达高水平的B-CAM。一个强的B-CAM阳性表型可以发现在成人和胎儿皮肤的空气毛囊的外根鞘以及在胎儿基底角质形成细胞。
The basal cell adhesion molecule (B-CAM) is a 90-kD cell surface glycoprotein with a characteristic immunoglobulin domain structure. The pattern of B-CAM expression in cultured cells suggests that the molecule is associated with a substrate-adherent growth pattern in some lineages. We investigated the expression of B-CAM in normal and diseased human epidermis by means of immunohistochemistry employing a single batch of high-titer mouse monoclonal antibody G253. Snap-frozen biopsy material from normal skin (n=8), psoliasis (n=5), contact dermatitis (n=6). basal cell carcinoma (n=5) and fetal skin (n=6) was studied. In normal human skin. B-CAM was found in varying degrees throughout the epidermis with a preference for suprabasal expression, hair follicles were regularly of a B-CAM-positive phenotype. There were no qualitative differences with regard to the B-CAM expression pattern in normal skin ill comparison to psoriasis and contact dermatitis. In contrast, fetal skin (15th to 18th week of gestation) was characterized by B-CAM-positive cells in the basal layer of the epidermis as well as in the outer root sheath of hair follicles. Basal cell carcinomas also regularly expressed high levels of B-CAM. A strong B-CAM-positive phenotype can be found in the outer root sheath of air follicles of adult and fetal human skin as well as in fetal basal kerarinocytes.