MicroRNA390 Is Involved in Cadmium Tolerance and Accumulation in Rice.
MicroRNA390 Is Involved in Cadmium Tolerance and Accumulation in Rice.
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MicroRNA390参与水稻的镉耐受和积累
DOI：
10.3389/fpls.2016.00235
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhu C
中科院分区：
文献类型：
--
作者：
Ding Y;Ye Y;Jiang Z;Wang Y;Zhu C
镉（Cd）是一种对植物有毒的非必需重金属。microRNAs （miRNAs）是21个核苷酸的rna，在转录后水平上是基因表达的普遍调节剂。几种植物mirna，如miR390，在植物生长发育和对环境胁迫（包括重金属胁迫）的响应中起着至关重要的作用。在本研究中，水稻在Cd胁迫下成熟miR390的表达显著下调。结果表明，Cd对miR390靶基因OsSRK的诱导作用显著。与野生型相比，过表达miR390的转基因水稻对Cd的耐受性降低，Cd积累量增加。同时，OsSRK在35S:MIR390植株中的表达也低于野生型。上述结果表明，miR390是水稻抗镉胁迫的负调控因子。
Cadmium (Cd) is a non-essential heavy metal that is toxic to plants. microRNAs (miRNAs) are 21-nucleotide RNAs that are ubiquitous regulators of gene expression at the post-transcriptional level. Several plant miRNAs, such as miR390, have vital roles in plant growth, development and responses to environmental stresses including heavy metal stress. In this study, the expression of mature miR390 was significantly down-regulated under Cd stress in rice. Consequently, the target gene of miR390, OsSRK was dramatically induced by Cd treatment. Transgenic rice plants overexpressing miR390 displayed reduced Cd tolerance and higher Cd accumulation compared with wild-type plants. Simultaneously, expression of OsSRK was less pronounced in 35S:MIR390 plants than in wild-type. These results indicate that miR390 was a negative regulator involved in Cd stress tolerance in rice.