Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia
Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia
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DOI：
10.1038/ng986
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发表时间：
2002-11-01
期刊：
影响因子：
30.8
通讯作者：
Aksoy, S
中科院分区：
文献类型：
--
作者：
Akman, L;Yamashita, A;Aksoy, S
许多在整个发育周期依赖单一食物来源的昆虫都含有有益的微生物，这些微生物可以提供其限制饮食中缺乏的营养。采采蝇是非洲锥虫的传播媒介，仅以血液为食，并依靠一种这样的细胞内微生物来提供营养和繁殖力。经过数百万年与宿主共同进化的结果，这些共生者已经失去了在宿主昆虫细胞的庇护环境之外生存的能力。我们展示了 Wigglesworthia glevipalpis 的完整注释基因组，它由一条 697,724 个碱基对 (bp) 的染色体和一个名为 pWig1 的 5,200 bp 的小质粒组成。参与维生素代谢物生物合成的基因被保留，这显然对宿主的营养和繁殖力至关重要。出乎意料的是，这种专性微生物的基因组同时具有寄生微生物和自由生活微生物的特征，并且编码重要调节蛋白 DnaA 的基因却缺失。
Many insects that rely on a single food source throughout their developmental cycle harbor beneficial microbes that provide nutrients absent from their restricted diet. Tsetse flies, the vectors of African trypanosomes, feed exclusively on blood and rely on one such intracellular microbe for nutritional provisioning and fecundity. As a result of co-evolution with hosts over millions of years, these mutualists have lost the ability to survive outside the sheltered environment of their host insect cells. We present the complete annotated genome of Wigglesworthia glossinidia brevipalpis, which is composed of one chromosome of 697,724 base pairs (bp) and one small plasmid, called pWig1, of 5,200 bp. Genes involved in the biosynthesis of vitamin metabolites, apparently essential for host nutrition and fecundity, have been retained. Unexpectedly, this obligate's genome bears hallmarks of both parasitic and free-living microbes, and the gene encoding the important regulatory protein DnaA is absent.