Chemical characterization and biologic properties of lipopolysaccharide from Bacteroides gingivalis strains W50, W83, and ATCC 33277.
Chemical characterization and biologic properties of lipopolysaccharide from Bacteroides gingivalis strains W50, W83, and ATCC 33277.
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牙龈拟杆菌菌株 W50、W83 和 ATCC 33277 脂多糖的化学特征和生物学特性。
DOI：
10.1111/j.1399-302x.1989.tb00250.x
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发表时间：
1989
影响因子：
--
通讯作者：
Holt,SC
中科院分区：
文献类型：
--
作者：
Bramanti,TE;Wong,GG;Weintraub,ST;Holt,SC
比较了牙龈类杆菌W50、W83和ATCC 33277菌株的脂多糖的化学组成和部分生物学活性，以及该分子作为部分炎症反应的介导物的作用。其化学组成为：脂肪A 47-58%，碳水化合物5-10%，3-脱氧-2-辛酸0.05%，庚糖0.3%，己糖胺3.8-5.2%，磷酸盐2%。鼠李糖是主要的糖(26-36%)，其次是葡萄糖(18-34%)、半乳糖(18-25%)、甘露糖(9-12%)、氨基葡萄糖(7-11%)和半乳糖胺(2-5%)。主要脂肪酸为：13-甲基十四酸(42-45%)、3-羟基-十七酸(21-23%)、十六酸(16-19%)和12-甲基-14酸(6-8%)。SDS-PAGE和脱氧胆酸钠-PAGE表明，脂多糖是一种光滑的化学型。强毒株和无毒株之间的迁移模式也存在差异。1μg/mlLPS3.2~4.2ngPGE/ml上清液作用于C3H/母鸡巨噬细胞(MO)，为对照组的236.278%。C3 H/HEN和C3 H/HEJ M0在5 0/*g/m l脂多糖作用下，IL-1活性分别为对照组的3 82~72 4%和2 70~30 0%，10μ/m l脂多糖作用于类似的Mo，分别释放1.6~2.0 ng/m l和0.3~0.5 ng/m l的肿瘤坏死因子。C3H/HEN M0细胞在50μg/ml脂多糖作用下最大释放肿瘤坏死因子，并可持续96h。这些结果表明，B细胞产生的内毒素。牙周炎菌株刺激M0产生细胞因子，而M0又可能在牙周疾病的发展中起到协调炎症反应的作用。
The chemistry and selected biological activity of lipopolysaccharide (LPS) fromBacteroides gingivalisstrains W50, W83, and ATCC 33277 were compared, as well as the role of this molecule as a mediator of selected inflammatory responses. Chemically, the LPSs consisted of 47–58% Lipid A, 5–10% carbohydrate, 0.05% 3‐deoxy 2‐octulosonic acid, 0.3% heptose, 3.8–5.2% hexosamine, and 2% phosphate. Rhamnose represented the dominant sugar (26–36%), with lesser amounts of glucose (18–34%), galactose (18–25%), mannose (9–12%), glucosamine (7–11%), and galaclosamine (2–5%). The major fatty acids were: 13‐methyl‐tetradecanoate (42–45%), 3‐OH‐heptadecanoate (21–23%), hexadecanoate (16–19%), and 12‐methyl‐tetradecanoate (6–8%). SDS‐PAGE and sodium deoxy‐cholate‐PAGE revealed the LPS to be a smooth chemotype. Differences in migration patterns between the virulent and avirulent strain LPSs also occurred. C3H/HeN macrophages (Mo) exposed to 1 μg/ml of LPS released 3.2–4.2 ng of prostaglandin E (PGE)/ml of supernatant, representing 236–278% of control. Interleukin‐1 (IL‐1) activity in C3H/HeN and C3H/HeJ M0 exposed to 50 /*g of LPS/ml was 382–724% and 270–300% of control, respectively; similar Mo exposed to 10 μg of LPS/ml released 1.6–2.0 ng and 0.3–0.5 ng of tumor necrosis factor (TNF)/ml of supernatant, respectively. Maximum TNF release in C3H/HeN M0 occurred in response to 50 μg of LPS/ml, and was sustained for up to 96 hours. These results suggest that LPS from theB. gingivalisstrains stimulate cytokine production from M0 which, in turn, may play a role in orchestrating the inflammatory response for the development of periodontal diseases.