Control of G2/M transition by Drosophila Fos
Control of G2/M transition by Drosophila Fos
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DOI：
10.1128/mcb.02455-05
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发表时间：
2006-11-01
影响因子：
5.3
通讯作者：
Bohmann, Dirk
中科院分区：
文献类型：
--
作者：
Hyun, Joogyung;Becam, Isabelle;Bohmann, Dirk
Fos家族的转录因子长期以来一直与细胞增殖的控制有关，尽管对介导这种功能的分子和细胞机制知之甚少。我们调查的贡献Fos的细胞周期和细胞生长控制使用果蝇成虫盘作为一个遗传可及的系统。RNA干扰介导的Fos抑制翅和眼盘增殖细胞中的Fos导致G（2）-到-M-期转变的特定缺陷，而细胞生长保持不受损害，导致器官大小显著减小。与Fos是有丝分裂所必需的结论一致，我们确定了细胞周期蛋白B作为果蝇中Fos的直接转录靶点，Fos在体内与细胞周期蛋白B基因上游区域结合，并且在Fos功能丧失条件下细胞周期蛋白B mRNA特异性降低。
The transcription factors of the Fos family have long been associated with the control of cell proliferation, although the molecular and cellular mechanisms that mediate this function are poorly understood. We investigated the contributions of Fos to the cell cycle and cell growth control using Drosophila imaginal discs as a genetically accessible system. The RNA interference-mediated inhibition of Fos in proliferating cells of the wing and eye discs resulted in a specific defect in the G(2)-to-M-phase transition, while cell growth remained unimpaired, resulting in a marked reduction in organ size. Consistent with the conclusion that Fos is required for mitosis, we identified cyclin B as a direct transcriptional target of Fos in Drosophila melanogaster, with Fos binding to a region upstream of the cyclin B gene in vivo and cyclin B mRNA being specifically reduced under Fos loss-of-function conditions.