Multilaboratory evaluation of an ultrafiltration procedure for high density lipoprotein cholesterol quantification in turbid heparin-manganese supernates.
Multilaboratory evaluation of an ultrafiltration procedure for high density lipoprotein cholesterol quantification in turbid heparin-manganese supernates.
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发表时间：
1981-08
影响因子：
6.5
通讯作者：
G. Warnick;J. Albers;Bachorik Ps;J. Turner;C. Garcia;C. Breckinridge;K. Kuba;S. McNeely;G. Hillerman;P. King;R. Muesing;B. Most;K. Lippel
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作者：
G. Warnick;J. Albers;Bachorik Ps;J. Turner;C. Garcia;C. Breckinridge;K. Kuba;S. McNeely;G. Hillerman;P. King;R. Muesing;B. Most;K. Lippel
高密度脂蛋白（HDL）可以通过用肝素和Mn（2+）沉淀低密度脂蛋白和极低密度脂蛋白（LDL和VLDL）后测量上清液中的胆固醇来定量。通常在高胆固醇血症标本中观察到的上清液浊度表明LDL/VLDL的不完全沉降，并妨碍HDL的准确定量。Ten Lipid Research Clinic Laboratories比较了用于清除浑浊肝素-Mn（2+）上清液的超滤技术与当前方法，后者涉及稀释后原始样本或通过超离心法从初始样本中去除VLDL后d> 1.006 g/ml的部分的重复沉淀。429份浑浊上清液的超滤结果平均仅略高于稀释法或超浓缩法对相同标本的结果（1.0 - 1.1 mg/dl），但发现这种差异具有显著性（P <0.005）。通过以下线性回归方程表明超滤法与其他两种方法的一致性：a），超滤=（0.964 x超滤）+2.4 mg/dl，相关系数= 0.926;和B），超滤=（0.936 x稀释）+3.3 mg/dl，相关系数= 0.933。我们的结论是，在稀释或去除VLDL后，对浑浊的肝素-Mn（2+）上清液进行超滤是沉淀的一种方便的替代方法。Warnick，G. R.，J. J.阿尔伯斯，P. Bachorik，J. Turner，C.加西亚角，澳-地布雷肯里奇湾Kuba，S. McNeely，G. Hillerman，P. King，R.穆辛，B。Most和K.利波多个实验室对肝素锰上清液中高密度脂蛋白胆固醇定量的超滤程序的评价。
High density lipoprotein (HDL) can be quantitated by measurement of cholesterol in supernates after precipitation of low and very low density lipoprotein (LDL and VLDL) with heparin and Mn(2+). Supernatant turbidity, often observed with hypertriglyceridemic specimens, indicates incomplete sedimentation of LDL/VLDL and precludes accurate quantitation of HDL. Ten Lipid Research Clinic Laboratories compared an ultrafiltration technique for clearing turbid heparin-Mn(2+) supernates to current methods involving repeat precipitation of either the original specimen after dilution or the d > 1.006 g/ml fraction after removal of VLDL from the initial specimen by ultracentrifugation. Results for ultrafiltration of 429 turbid supernates averaged only slightly higher (1.0-1.1 mg/dl) than results by the dilution or ultracentrifugation methods on the same specimens, but this difference was found to be significant (P < 0.005). The agreement of the ultrafiltration method with the other two methods is indicated by the following linear regression equations: a), ultrafiltration = (0.964 x ultracentrifugation) + 2.4 mg/dl, and correlation coefficient = 0.926; and b), ultrafiltration = (0.936 x dilution) + 3.3 mg/dl, and correlation coefficient = 0.933. We conclude that ultrafiltration of turbid heparin-Mn(2+) supernates is a convenient alternative to precipitation after either dilution or removal of VLDL.-Warnick, G. R., J. J. Albers, P. Bachorik, J. Turner, C. Garcia, C. Breckinridge, K. Kuba, S. McNeely, G. Hillerman, P. King, R. Muesing, B. Most, and K. Lippel. Multi-laboratory evaluation of an ultrafiltration procedure for high density lipoprotein cholesterol quantification in turbid heparin-manganese supernates.