Identification and Characterization of Human snoRNA Core Promoters
Identification and Characterization of Human snoRNA Core Promoters
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人类 snoRNA 核心启动子的鉴定和表征
DOI：
10.1016/j.ygeno.2010.03.010
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发表时间：
2010-07-01
期刊：
影响因子：
4.4
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Li, Tingting;Zhou, Xueya;Zhang, Yong
核仁小RNA（snoRNAs）是目前在真核细胞中已知的最大的功能性非蛋白质编码RNA群体之一。内含子编码的snoRNAs的加工过程已有详尽记载；然而，snoRNA基因的转录调控仍知之甚少，很可能是由于缺乏对snoRNA启动子的特性描述。在此我们使用一种计算方法来预测131种人类snoRNAs的核心启动子。大多数假定的snoRNA启动子得到了DNA酶I超敏性、RNA聚合酶II染色质免疫沉淀测序峰或基因表达系列分析（CAGE）标签簇的支持。基于预测的人类snoRNA启动子的基因组结构，我们提出了这些snoRNA基因的五种转录模式；我们还发现有证据表明一些人类内含子snoRNAs可能拥有自身的启动子。本研究是首次对人类snoRNAs启动子进行的计算机模拟筛选；我们预期这些数据将促进对人类snoRNA基因转录控制的进一步分子特性描述。（C）2010爱思唯尔公司。保留所有权利。
Small nucleolar RNAs (snoRNAs) represent one of the largest groups of functional non-protein coding RNAs currently known in eukaryotic cells. The processing of intron encoded snoRNAs has been well documented; however, the transcriptional regulation of snoRNA genes is still poorly understood, most likely due to the lack of characterization of snoRNA promoters. Here we used a computational approach to predict core promoters for 131 human snoRNAs. Majority of putative snoRNA promoters are supported by DNase I hypersensitivities, RNA polymerase II ChIP-seq peaks, or CAGE tag clusters. Based on the genomic organizations of predicted human snoRNA promoters, we propose five transcriptional models of those snoRNA genes; we also found evidence that some intronic human snoRNAs might have their own promoters. The present study is the first in silico screening of human snoRNAs promoters; and we anticipate the data will facilitate further molecular characterization of transcriptional control of human snoRNA genes. (C) 2010 Elsevier Inc. All rights reserved.