Species differences in the distribution of gamma-glutamyl transpeptidase in choroid plexus of lateral ventricle and microvessels of adjacent brain.
Species differences in the distribution of gamma-glutamyl transpeptidase in choroid plexus of lateral ventricle and microvessels of adjacent brain.
复制标题
侧脑室脉络丛及邻近脑微血管中γ-谷氨酰转肽酶分布的物种差异。
DOI：
10.1007/bf00160058
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Mitro,A
中科院分区：
文献类型：
--
作者：
DeBault,LE;Mitro,A
采用酶组织化学方法，研究了γ-谷氨酰转肽酶在仓鼠、沙鼠、豚鼠、大鼠和小鼠等几种常见实验动物中枢神经系统血管间室中的分布。5种动物脑室周围脑组织微血管内皮均呈阳性染色。沙鼠脉络膜丛血管内皮染色呈阳性，其余4种均呈阴性。在豚鼠、大鼠和小鼠的脉络膜丛上皮细胞中也发现转肽酶阳性反应；但在仓鼠和沙鼠的这些细胞中未发现活性。结果表明，γ-谷氨酰转肽酶在脉络膜丛中促进氨基酸转运的作用在不同动物中存在明显的物种差异。在沙鼠中，转肽酶辅助氨基酸转运发生在脉络膜丛内皮细胞中，而在豚鼠、大鼠和小鼠中，转运发生在脉络膜丛上皮细胞中。在仓鼠的情况下，这种辅助运输在脉络膜丛的内皮细胞和上皮细胞中是不存在的。因此，仓鼠和沙鼠在γ-谷氨酰转肽酶分布上存在差异，而豚鼠、大鼠和小鼠在γ-谷氨酰转肽酶分布上具有相似性。
The distribution of γ-glutamyl transpeptidase in different vascular compartments of the central nervous system was evaluated in several common laboratory Animals, i.e., hamster, gerbil, guinea pig, rat and mouse, by enzyme-histochemistry. Microvascular endothelium of the periventricular brain tissue stained positively in all five species. In contrast, the vascular endothelium of the choroid plexus stained positively only in the gerbil, and was negative in the other four species. Positive reactions for the transpeptidase was also found in choroid plexus epithelial cells in guinea pig, rat, and mouse; however no activity could be demonstrated in these cells of hamster and gerbil.The results demonstrate clear species differences in localization of the enzyme and suggest that γ-glutamyl transpeptidase-promoted amino acid transport in choroid plexus is different in various animal species. It is also suggested that in gerbil, transpeptidase-aided amino acid transport takes place in endothelial cells of choroid plexus, whereas in guinea pig, rat and mouse this occurs in epithelial cells of choroid plexus. In the case of hamster, such aided transport is absent in endothelial as well as in epithelial cells of the choroid plexus. Thus, the hamster and the gerbil showed differences in γ-glutamyl transpeptidase distribution, whereas the guinea pig, rat, and mouse showed similar enzyme distributions.