Differential expression of basement membrane type IV collagen alpha chains in gastric intramucosal neoplastic lesions.
Differential expression of basement membrane type IV collagen alpha chains in gastric intramucosal neoplastic lesions.
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发表时间：
2007
影响因子：
6.3
通讯作者：
Y. Baba;Ken-ichi Iyama;Koei Ikeda;Shinji Ishikawa;Naoko Hayashi;N. Miyanari;Yumi Honda;Y. Sado;Y. Ninomiya;Hideo Baba
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文献类型：
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作者：
Y. Baba;Ken-ichi Iyama;Koei Ikeda;Shinji Ishikawa;Naoko Hayashi;N. Miyanari;Yumi Honda;Y. Sado;Y. Ninomiya;Hideo Baba
背景胃粘膜内肿瘤性病变（GINLs）的组织学诊断在有经验的病理学家中存在争议。尽管上皮基底膜（BM）的破坏和肿瘤上皮细胞侵入固有层的粘膜是“粘膜内癌”的明显证据，但GINL的组织学评价是困难和模糊的，特别是膀胱型腺癌。IV型胶原蛋白是BM的主要成分，并且包含六条遗传上不同的α（IV）链，α 1（IV）至α 6（IV）。在几种类型的癌中，已经报道了在早期侵袭阶段上皮BM的α 5/α 6（IV）链的损失。然而，α 5/α 6（IV）链在GINL中的表达仍不清楚。我们检测了GINLs中α（IV）链的免疫组化表达，并研究其表达模式是否是胃粘膜内癌的诊断标志物。方法采用免疫组织化学方法检测60例GINL组织中α（IV）链和Ki-67的表达。结果在正常胃粘膜中，α 1（IV）、α 2（IV）、α 5（IV）和α 6（IV）链在BM中连续表达。在大多数管状腺瘤（日本分类）中，这四条链在BM中连续染色，而在管状腺癌（日本分类）中，α 5/α 6（IV）链部分或完全消失。α 5/α 6（IV）链的表达与组织学分级密切相关。此外，α 5/α 6（IV）链的丢失与肿瘤细胞生长活性显著相关。结论α 5/α 6（IV）链缺失可能是GINL患者胃粘膜内癌的一个有用的诊断指标。
BACKGROUND The histological diagnosis of gastric intramucosal neoplastic lesions (GINLs) is controversial among experienced pathologists. Although the destruction of the epithelial basement membrane (BM) and the invasion of neoplastic epithelial cells into the interstitium of the lamina propria is distinct proof of "intramucosal carcinoma," histological evaluation of GINLs is difficult and ambiguous, especially intestinal-type adenocarcinoma. Type IV collagen is a major component of the BM, and comprises six genetically distinct alpha(IV) chains, alpha1(IV) to alpha6(IV). In several types of carcinomas, the loss of alpha5/alpha6(IV) chains of the epithelial BM at an early invasive stage has been reported. However, the expression of alpha5/alpha6(IV) chains in GINLs is still unclear. We examined the immunohistochemical expression of alpha(IV) chains in GINLs and investigated whether the expression pattern was a diagnostic marker of gastric intramucosal carcinoma. METHODS The expression of alpha(IV) chains and Ki-67 in 60 resected GINL specimens was immunohistochemically examined. RESULTS In normal gastric epithelium, alpha1(IV), alpha2(IV), alpha5(IV), and alpha6(IV) chains were expressed continuously in the BM. In most tubular adenomas (Japanese classification), these four chains were stained continuously in the BM, whereas in tubular adenocarcinomas (Japanese classification), the alpha5/alpha6(IV) chains had disappeared, partially or completely. The expression of alpha5/alpha6(IV) chains was closely related to the grade of histological atypia. In addition, the loss of alpha5/alpha6(IV) chains was significantly correlated with tumor cell growth activity. CONCLUSIONS The loss of alpha5/alpha6(IV) chains might be a useful diagnostic finding for gastric intramucosal carcinoma in GINL cases.