Experimentally determined sequence requirement of ACGT-containing abscisic acid response element
Experimentally determined sequence requirement of ACGT-containing abscisic acid response element
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DOI：
10.1093/pcp/pcf014
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发表时间：
2002-01-01
影响因子：
4.9
通讯作者：
Yamamoto-Toyoda, A
中科院分区：
文献类型：
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作者：
Hattori, T;Totsuka, M;Yamamoto-Toyoda, A
通过系统地替换基序 A 的 ACGF 核心周围的碱基，分析了含有 ACGT 的脱落酸反应元件 (ABRE) 的序列要求，基序 A 是水稻基因 Osem 的主要 ABRE。这是在 55 bp 启动子片段的背景下完成的，该片段最低限度地赋予异源启动子 ABA 响应性。基于此分析，含有 ACGT 的 ABRE 的序列要求被确定为 ACGTG G/T C，这与 ABA 响应启动子序列比较得出的共识非常匹配。
The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGF-core of motif A, the principal ABRE of the rice gene, Osem. This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.