Plant volatile ligands for male-biased MmedOBP14 stimulate orientation behavior of the parasitoid wasp Microplitis mediator.
Plant volatile ligands for male-biased MmedOBP14 stimulate orientation behavior of the parasitoid wasp Microplitis mediator.
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DOI：
10.1016/j.ijbiomac.2022.11.149
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发表时间：
2022-11
影响因子：
8.2
通讯作者：
Ruijun Li;S. Shan;Xuan Song;A. Khashaveh;Shan-Ning Wang;Zixuan Yin;Ziyun Lu;K. H. Dhiloo;Yongjun Zhang
中科院分区：
文献类型：
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作者：
Ruijun Li;S. Shan;Xuan Song;A. Khashaveh;Shan-Ning Wang;Zixuan Yin;Ziyun Lu;K. H. Dhiloo;Yongjun Zhang
气味结合蛋白（odorant binding proteins，OBP）是一类重要的化学感觉相关蛋白，在昆虫嗅觉信号的感知中起着重要作用。寄生蜂Microplitismediator依靠其敏感的嗅觉系统来定位夜蛾科和尺蛾科的寄主幼虫。在本研究中，MmedOBP 14，一个男性偏向的OBP在M。调解人，进行了功能调查。在荧光竞争结合实验中，重组MmedOBP 14对β-紫罗兰酮、3，4-二甲基苯乙酮、4-乙基苯乙酮、苯乙酮和罗勒烯等5种植物挥发物表现出较强的结合能力。同源模建和分子对接结果表明，所有5个配体的结合位点是相似的，集中在MmedOBP 14的结合口袋。除苯乙酮外，其余4种配体在1、10和100 μg/μL浓度下均能引起成虫的强烈触角电生理反应。对大多数配体的EAG反应，雄性比雌性明显。在行为试验中，雄性被低浓度的MmedOBP 14配体吸引，而高剂量的β-紫罗兰酮和苯乙酮对雄性有驱避作用。1 μg/μL的3，4-二甲基苯乙酮对雌蜂的引诱力最强。这些发现表明MmedOBP 14可能在雄蜂感知植物挥发物以定位栖息地、补充营养和寻找伴侣方面发挥更重要的作用。
As an important class of chemosensory-associated proteins, odorant binding proteins (OBPs) play a key role in the perception of olfactory signals for insects. Parasitoid waspMicroplitismediatorrelies on its sensitive olfactory system to locate host larvae of Noctuidae and Geometridae. In the present study, MmedOBP14, a male-biased OBP inM. mediator, was functionally investigated. In fluorescence competitive binding assays, the recombinant MmedOBP14 showed strong binding abilities to five plant volatiles: β-ionone, 3,4-dimethylacetophenone, 4-ethylacetophenone, acetophenone and ocimene. Homology modeling and molecular docking results indicated that the binding sites of all five ligands were similar and concentrated in the binding pocket of MmedOBP14. Except acetophenone, the remaining four ligands at 1, 10 and 100 μg/μL caused strong antennal electrophysiological responses in adultsM. mediator, and males showed more obvious EAG responses to most ligands than females. In behavioral trials, males were attracted by low concentrations of MmedOBP14 ligands, whereas high doses of β-ionone and acetophenone had a repellent effect on males. Moreover, 1 μg/μL of 3,4-dimethylacetophenone showed the strongest attractiveness to female wasps. These findings suggest that MmedOBP14 may play a more important role in the perception of plant volatiles for male wasps to locate habitat, supplement nutrition and search partners.