Susceptibility of Culicoides biting midge larvae to the insect-pathogenic fungus, Metarhizium anisopliae: Prospects for bluetongue vector control
Susceptibility of Culicoides biting midge larvae to the insect-pathogenic fungus, Metarhizium anisopliae: Prospects for bluetongue vector control
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DOI：
10.1016/j.actatropica.2009.08.022
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发表时间：
2010-01-01
期刊：
影响因子：
2.7
通讯作者：
Butt, T. M.
中科院分区：
文献类型：
--
作者：
Ansari, M. A.;Carpenter, S.;Butt, T. M.
库蚊是具有国际重要性的多种家畜和人类虫媒病毒传播的罪魁祸首。本研究测定了来自绿僵菌属、白僵菌属、板蓝根霉属和链球菌属的18株致病真菌对库蚊幼虫期的毒力，以此作为检测它们作为生防剂的可能性的手段。在初步筛选中，6株金龟子绿僵菌(ERL700、CA1、V275、LRC181A、ARSEF3291和ARSEF4556)的表现优于其他供试属，接种72h后，该物种所有幼虫的死亡率在90%~100%之间。用10(4)-10(8)孢子/毫升的剂量照射幼虫，在24孔培养板上记录幼虫在24、48和72小时的死亡率，每孔含有600亩L水，在250毫升塑料杯中记录24和48小时的死亡率，进一步测定最有效菌株V275的毒力。在多孔培养板上，幼虫的敏感度极高，LC_(50)为4.3-4.5×10~(3)孢子/毫升，不同龄期的幼虫之间无显著差异。在250ml杯中，绿僵菌V275对幼虫的致死率为70%~100%，后期幼虫的死亡率较高。讨论了将绿僵菌纳入生物防治计划以控制库蚊传播的虫媒病毒的结果。(C)2009爱思唯尔B.V.保留所有权利。
Culicoides biting midges (Diptera: Ceratopogonidae) are responsible for the spread of several arboviruses of livestock and humans that are of international importance. This study assesses the virulence of 18 insect-pathogenic fungal strains from the genera metarhizium, Beauveria, Isaria and Lecanicillium to larval stages of Culicoides nubeculous Meigen as a means of examining their potential as biocontrol agents. In initial screening, six strains of M. anisopliae (ERL700, CA1, V275, LRC181A, ARSEF 3291 and ARSEF 4556) outperformed the other tested genera and were found to cause between 90% and 100% larval mortality in all larval instars of this species at 72 h post inoculation. The virulence of the most effective strain, M. anisopliae V275, was then further tested by exposing larvae to doses which ranged from 10(4)-10(8) conidia/ml and recording mortality at 24, 48 and 72 h in a 24-multi-well plate with each well containing 600 mu l of water and at 24 and 48 h in 250 ml plastic cups containing 50 ml of water. Sensitivity of larvae was extremely high in the multi-well plates, with LC50 values of 4.3-4.5 x 10(3) conidia/ml and no significant differences between larval instars. In the 250 ml cups, M. anisopliae V275 caused mortalities of between 70% and 100% to larvae and later instars exhibited higher mortality rates. The results are discussed in relation to incorporation of M. anisopliae into biocontrol programmes to control arboviruses vectored by Culicoides. (C) 2009 Elsevier B.V. All rights reserved.