α-tocopherol, an exogenous factor of adult hippocampal neurogenesis regulation
α-tocopherol, an exogenous factor of adult hippocampal neurogenesis regulation
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DOI：
10.1002/jnr.10690
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发表时间：
2003-08-15
影响因子：
4.2
通讯作者：
Del Grande, P
中科院分区：
文献类型：
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作者：
Cecchini, T;Ciaroni, S;Del Grande, P
在之前的工作中，我们发现维生素E缺乏会影响成年大鼠海马神经发生。由于维生素 E 缺乏症是一种涉及众多生物系统的复杂病症，因此其对出生后新神经元生成的影响可能是由不同因素的未知变化介导的，而这些因素又在此过程中发挥作用。为了阐明维生素 E 是否在调节海马神经发生中发挥直接作用，我们研究了成年对照大鼠和补充 α-生育酚（维生素 E 最重要的化合物）的成年大鼠的神经发生。对对照大鼠和补充大鼠的 α-生育酚水平进行了监测。对海马齿状回细胞增殖和死亡进行定性和定量分析，并研究未成熟神经元标志物PSA-NCAM、TUC 4和DCX的表达。补充后，血浆和大脑中的α-生育酚水平显着增加。补充α-生育酚的大鼠的细胞增殖受到抑制，垂死细胞的数量减少，表达未成熟神经元标记物的细胞数量增加。所获得的结果证实并扩展了维生素E是一种外源性因子，在调节成人海马神经发生的不同步骤中发挥直接作用的观点。提出并讨论了关于 α-生育酚复杂作用的可能机制的一些假设，与其抗氧化和分子特异性非抗氧化特性有关。 (C) 2003 Wiley-Liss, Inc.
In previous work, we found that adult hippocampal neurogenesis in rat is affected by vitamin E deficiency. Because vitamin E deficiency is a complex condition involving numerous biological systems, it is possible that its effect on postnatal new neuron production could be mediated by unknown changes in different factors that in turn play a role in this process. To clarify if vitamin E plays a direct role in regulating hippocampal neurogenesis, we studied the neurogenesis in adult control rats and in adult rats under supplementation with alpha-tocopherol, the most important compound of vitamin E. The alpha-tocopherol level in control and supplemented rats was monitored. Qualitative and quantitative analysis of cell proliferation and death was carried out and expression of immature neuron markers PSA-NCAM, TUC 4, and DCX was investigated in hippocampus dentate gyrus. alpha-Tocopherol levels increased significantly in both plasma and brain after supplementation. Cell proliferation was inhibited in alpha-tocopherol-supplemented rats, the number of dying cells was reduced, and the number of cells expressing the immature neuron markers was increased. The results obtained confirm and extend the idea that vitamin E is an exogenous factor playing a direct role in regulation of different steps of adult hippocampal neurogenesis. Some hypotheses about the possible mechanisms underlying the complex action of alpha-tocopherol, related to its antioxidant and molecule-specific non-antioxidant properties, are proposed and discussed. (C) 2003 Wiley-Liss, Inc.