Isolation of rotaviruses from turkeys and chickens: demonstration of distinct serotypes and RNA electropherotypes.
Isolation of rotaviruses from turkeys and chickens: demonstration of distinct serotypes and RNA electropherotypes.
复制标题
从火鸡和鸡中分离轮状病毒：展示不同的血清型和 RNA 电泳型。
作者：
M. Mcnulty;G. Allan;Daniel A. Todd;J. Mcferran;E. McKillop;D. S. Collins;R. McCracken
从火鸡中分离出6株轮状病毒，从鸡中分离出2株。其中三个需要胰蛋白酶处理，用于分离和在细胞培养物中连续传代。其余的在没有胰蛋白酶处理的情况下分离。这些病毒大多数是从1周龄以下禽类粪便中的鸡胚肝细胞培养物中分离出来的。在8例病例中的6例中，轮状病毒分离与肠道紊乱相关，其特征为腹泻、食欲不振或异常、异常液体或气体肠内容物或死亡率增加等体征。交叉免疫荧光试验表明，虽然鸟类和哺乳动物轮状病毒共享一个共同的组抗原，鸟类病毒更密切相关的彼此比内布拉斯加州小牛轮状病毒分离。在血清中和试验的基础上，将8种禽轮状病毒中的7种分为3种血清型，其中2种火鸡分离株（Ty1和Ty3）和1种鸡（Ch1）病毒为原型株。剩余的病毒Ty2的类型介于Ty1和Ch1之间。用聚丙烯酰胺凝胶电泳对这些病毒的RNA进行分析，结果表明它们也可分为若干电泳型。血清学上不同的分离株在病原学上也不同。同样，属于Ty3血清型的5个分离株在病原学上是相同的。血清学和电泳差异的分析表明，RNA片段5可能编码的类型特异性抗原。
Six isolates of rotavirus were made from turkeys and two from chickens. Three of these required trypsin treatment for isolation and serial passage in cell cultures. The remainder were isolated without trypsin treatment. Most of these viruses were isolated in chick embryo liver cell cultures from the faeces of birds aged under 1 week. In six of the eight instances, rotavirus isolation was associated with enteric disturbance, characterised by signs such as diarrhoea, poor or abnormal appetite, abnormally fluid or gaseous intestinal contents or increased mortality. Cross immunofluorescence tests showed that while avian and mammalian rotaviruses shared a common group antigen, the avian viruses were more closely related to each other than to the Nebraska calf rotavirus isolate. On the basis of serum neutralisation tests seven of the eight avian rotaviruses were grouped into three serotypes, with two turkey isolates (Ty1 and Ty3) and a chicken (Ch1) virus being the prototype strains. The remaining virus, Ty2, was intermediate in type between Ty1 and Ch1. Analysis of the RNA of these viruses by polyacrylamide gel electrophoresis showed that they could also be grouped into a number of electropherotypes. The isolates which were serologically distinct were also electrophoretically distinct. Similarly the five isolates which belonged to the Ty3 sero-type were electrophoretically identical. Analysis of the serological and electrophoretic differences suggested that RNA segment 5 may code for a type-specific antigen.