Evolution and Dynamic Transcriptome of Key Genes of Photoperiodic Flowering Pathway in Water Spinach (Ipomoea aquatica)
Evolution and Dynamic Transcriptome of Key Genes of Photoperiodic Flowering Pathway in Water Spinach (Ipomoea aquatica)
复制标题
DOI：
10.3390/ijms25031420
复制
发表时间：
2024-01
影响因子：
5.6
通讯作者：
Xin Wang;Yuanyuan Hao;Muhammad Ahsan Altaf;Huangying Shu;Shanhan Cheng;Zhiwei Wang;Guopeng Zhu
中科院分区：
文献类型：
--
作者：
Xin Wang;Yuanyuan Hao;Muhammad Ahsan Altaf;Huangying Shu;Shanhan Cheng;Zhiwei Wang;Guopeng Zhu
光周期是控制开花的主要环境因子。空心菜在诱导短日照条件下开花会降低营养组织产量和食味品质。为了深入了解光周期依赖性调控空心菜开花时间的分子机制，我们在长日照和短日照条件下对空心菜进行了8个时间点的转录组测序。结果表明，长日照条件下与昼夜节律相关的基因有6615个，短日照条件下有8691个。三个关键的昼夜节律基因，IaCCA1，IaLHY和IaTOC1，仍然保持单拷贝和类似的IaCCA1，IaLHY和IaTOC1反馈表达模式，表明反向反馈的保守性。在光周期途径中，空心菜中高度保守的GI基因扩增为两个拷贝（IaGI 1和IaGI 2）。两个基因表达的显著差异表明功能多样性。虽然光周期核心基因FT在空心菜中复制为3个拷贝，但只有IaFT 1在空心菜中高表达，并对光周期和昼夜节律反应强烈，IaFT 1在空心菜中几乎完全被抑制可能是空心菜无论在长日照条件下持续多长时间都不开花的原因。与其他物种不同的是（1）尼勒岛特里洛巴岛Ipomoea属的C.trifida）具有三个CO成员，空心菜缺少其中一个，并且另外两个CO基因（IaCO 1和IaCO 2）仅编码一个CCT结构域。此外，通过加权相关网络分析（WGCNA），获得了一些与光周期途径密切相关的转录因子。本研究为进一步深入分析空心菜和空心菜属植物开花时间的分子调控提供了有价值的数据。
The photoperiod is a major environmental factor in flowering control. Water spinach flowering under the inductive short-day condition decreases the yield of vegetative tissues and the eating quality. To obtain an insight into the molecular mechanism of the photoperiod-dependent regulation of the flowering time in water spinach, we performed transcriptome sequencing on water spinach under long- and short-day conditions with eight time points. Our results indicated that there were 6615 circadian-rhythm-related genes under the long-day condition and 8691 under the short-day condition. The three key circadian-rhythm genes, IaCCA1, IaLHY, and IaTOC1, still maintained single copies and similar IaCCA1, IaLHY, and IaTOC1 feedback expression patterns, indicating the conservation of reverse feedback. In the photoperiod pathway, highly conserved GI genes were amplified into two copies (IaGI1 and IaGI2) in water spinach. The significant difference in the expression of the two genes indicates functional diversity. Although the photoperiod core gene FT was duplicated to three copies in water spinach, only IaFT1 was highly expressed and strongly responsive to the photoperiod and circadian rhythms, and the almost complete inhibition of IaFT1 in water spinach may be the reason why water spinach does not bloom, no matter how long it lasts under the long-day condition. Differing from other species (I. nil, I. triloba, I. trifida) of the Ipomoea genus that have three CO members, water spinach lacks one of them, and the other two CO genes (IaCO1 and IaCO2) encode only one CCT domain. In addition, through weighted correlation network analysis (WGCNA), some transcription factors closely related to the photoperiod pathway were obtained. This work provides valuable data for further in-depth analyses of the molecular regulation of the flowering time in water spinach and the Ipomoea genus.