CNS GABA neurons express the mu-opioid receptor: immunocytochemical studies
CNS GABA neurons express the mu-opioid receptor: immunocytochemical studies
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DOI：
10.1097/00001756-199710200-00035
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发表时间：
1997-10-20
期刊：
影响因子：
1.7
通讯作者：
Wessendorf, MW
中科院分区：
文献类型：
--
作者：
Kalyuzhny, AE;Wessendorf, MW
多阿片受体通过降低gaba能张力来刺激海马和中缝背核（NRD）神经元。在本研究中，我们研究了gaba能神经元和mu-阿片受体之间相互作用的免疫细胞化学证据是否可以在中枢神经系统中找到。采用双色免疫荧光法对大鼠脑部分进行GABA和克隆的mu-阿片受体（MOR1）染色。GABA和MOR1双标记的神经元存在于海马和NRD，以及嗅球、背外侧导水管周围灰质、中缝正中核、中缝暗核和脊髓三叉神经核和束。我们得出结论，gaba能神经元表达mu-阿片受体在大鼠中枢神经系统中是常见的。
IT has been proposed that mu-opioid receptors excite neurons in hippocampus and nucleus raphe dorsalis (NRD) by decreasing GABAergic tone. In the present study, we examined whether immunocytochemical evidence of interaction between GABAergic neurons and the mu-opioid receptor could be found in the CNS. Portions of rat brain were sectioned and stained for GABA and for the cloned mu-opioid receptor (MOR1) using two-color immunofluorescence. Neurons double-labeled for GABA and MOR1 were present in hippocampus and NRD, as well as in olfactory bulb, dorsal lateral periaqueductal gray matter, nucleus raphe medianis, nucleus raphe obscurus, and the spinal trigeminal nucleus and tract. We conclude that expression of the mu-opioid receptor by GABAergic neurons is common in the rat CNS.