Accumulation and release of isoferritins during incubation in vitro of human peripheral blood mononuclear cells
Accumulation and release of isoferritins during incubation in vitro of human peripheral blood mononuclear cells
复制标题
人外周血单核细胞体外培养过程中异铁蛋白的积累和释放
DOI：
10.1111/j.1365-2141.1984.tb01269.x
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发表时间：
1984
影响因子：
6.5
通讯作者：
B. Jones
中科院分区：
文献类型：
--
作者：
M. Worwood;D. Hourahane;B. Jones
在体外培养后的外周血单个核细胞和培养液中测量了铁蛋白浓度。使用心脏和脾脏铁蛋白抗体。在含铁约12 μmol /1的培养基中培养的单核细胞铁蛋白积累迅速，心脾铁蛋白比由3:1增加到约10:1。更高浓度的铁（100 μmol/1）产生更大的效果。在培养培养基中加入去铁胺（2 mmol/1）可防止铁蛋白的积累。铁蛋白的积累似乎主要发生在单核细胞中。红细胞的吞噬也会引起铁蛋白的快速积累，但心脾比没有任何变化。少量脾型和心型铁蛋白在含铁培养基中孵育期间和红细胞吞噬后释放。一些豆豆蛋白A结合铁蛋白也被释放，这表明吞噬细胞可能是正常血浆中发现的豆豆蛋白A结合铁蛋白的来源。
Summary Ferritin concentration has been measured in peripheral blood mononuclear cells and in the incubation medium following in vitro culture. Antibodies to both heart and spleen ferritin were used. Mononuclear cells cultured in medium containing about 12 μmol Fe/1 accumulate ferritin rapidly with an increase in the heart:spleen ferritin ratio from 3:1 to about 10:1. Higher concentrations of iron (100 μmol/1) produce an even greater effect. The accumulation of ferritin is prevented by the addition of desferrioxamine (2 mmol/1) to the incubation medium. Accumulation of ferritin appears to take place largely in monocytes. Phagocytosis of red blood cells also causes rapid accumulation of ferritin but without any change in the heart: spleen ratio. Small amounts of both spleen and heart type ferritin are released during incubation in an iron containing medium and following phagocytosis of red blood cells. Some concanavalin A binding ferritin is also released suggesting that phagocytic cells may be a source of the concanavalin A binding ferritin found in normal plasma.