Evolution of the chitin synthase gene family correlates with fungal morphogenesis and adaption to ecological niches.
Evolution of the chitin synthase gene family correlates with fungal morphogenesis and adaption to ecological niches.
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几丁质合酶基因家族的进化与真菌形态发生和对生态位的适应相关
DOI：
10.1038/srep44527
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发表时间：
2017-03-16
影响因子：
4.6
通讯作者：
Fang W
中科院分区：
文献类型：
--
作者：
Liu R;Xu C;Zhang Q;Wang S;Fang W
真菌界可能拥有最复杂的几丁质合成酶（CHS）基因家族，但真菌CHS基因家族的进化及其多样化以实现多种功能仍有待阐明。在这里，我们从231种真菌中鉴定了CHSs的完整补体。利用迄今为止最大的数据集，我们利用系统发育和结构域分析表征了真菌CHS基因家族的进化。基因复制、结构域重组和增生是真菌CHS基因家族多样化的主要机制，产生了至少7个CHS类。CHS基因家族的收缩是形态特异性的，在单细胞真菌中有明显的损失，而家族扩张是谱系特异性的，在早期分化真菌中有明显的扩张。通过募集PF00063和PF08766结构域及随后的重复产生具有相同结构域结构的第v类和第vii类chs。通过对其在多种真菌物种中的功能对比分析，表明v类和vii类CHSs的出现对丝状真菌的形态发生、致病真菌的致病性发展以及Pezizomycotina真菌的耐热性具有重要意义。本研究揭示了真菌CHS基因家族的进化及其与真菌形态发生和生态位适应的关系。
The fungal kingdom potentially has the most complex chitin synthase (CHS) gene family, but evolution of the fungal CHS gene family and its diversification to fulfill multiple functions remain to be elucidated. Here, we identified the full complement of CHSs from 231 fungal species. Using the largest dataset to date, we characterized the evolution of the fungal CHS gene family using phylogenetic and domain structure analysis. Gene duplication, domain recombination and accretion are major mechanisms underlying the diversification of the fungal CHS gene family, producing at least 7 CHS classes. Contraction of the CHS gene family is morphology-specific, with significant loss in unicellular fungi, whereas family expansion is lineage-specific with obvious expansion in early-diverging fungi. ClassV and ClassVII CHSs with the same domain structure were produced by the recruitment of domains PF00063 and PF08766 and subsequent duplications. Comparative analysis of their functions in multiple fungal species shows that the emergence of ClassV and ClassVII CHSs is important for the morphogenesis of filamentous fungi, development of pathogenicity in pathogenic fungi, and heat stress tolerance in Pezizomycotina fungi. This work reveals the evolution of the fungal CHS gene family, and its correlation with fungal morphogenesis and adaptation to ecological niches.