Immunogenicity of various presentation forms of PorA outer membrane protein of Neisseria meningitidis in mice
Immunogenicity of various presentation forms of PorA outer membrane protein of Neisseria meningitidis in mice
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DOI：
10.1016/s0264-410x(99)00011-0
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发表时间：
1999-06-04
期刊：
影响因子：
5.5
通讯作者：
Poolman, JT
中科院分区：
文献类型：
--
作者：
Peeters, CCAM;Claassen, IJTM;Poolman, JT
在这项研究中，我们比较了含有脑膜炎双球菌PporA外膜蛋白、纯化的PporA、外膜囊泡(OMV)和免疫刺激复合体(ISCOM)的不同疫苗配方。OMV和ISCOM制剂可以产生杀菌抗体，但用AlPO4或Quil-A作为佐剂的纯化PORA不能产生杀菌抗体。OMV和ISCOM制剂在杀菌和OMV结合抗体方面以剂量反应方式进行测试时，显示出类似的免疫原性。OMV制剂中PORA诱导的抗OMV Ig G亚类反应在所有Ig G1、Ig G2a、Ig C2b、Ig G3、OMP-iscoms诱导的高抗OMV抗体中均可见，但几乎无Ig G3反应。此外，OMP iscoms似乎是针对PORA表面暴露环对应的线状肽的抗体的有效诱导者。此外，ISCOMS和以Quil-A为佐剂(但不含AlPO4)的纯化PORA均可诱导高水平的抗体产生。综上所述，除了OMV制剂外，只有含有PORA的ISCOMS能够产生回忆和杀菌抗体反应。(C)1999由爱思唯尔科学有限公司出版。版权所有。
In this study we compare different vaccine formulations containing meningococcal PorA outer membrane protein; purified PorA, outer membrane vesicles (OMV) and immune-stimulating complexes (iscom). Bactericidal antibodies could be generated by the OMV and iscom formulation but not with purified PorA using either AlPO4 or Quil-A as adjuvant. OMV and iscom formulations revealed similar immunogenicity when tested in a dose response manner, with respect to bactericidal as well as OMV-binding antibodies. The anti-OMV IgG subclass response induced by PorA in OMV formulation was found in all subclasses IgG1, IgG2a, IgC2b, IgG3, OMP-iscoms induced very high IgG1 anti-OMV antibodies but almost no IgG3 response. Also, OMP iscoms appeared to be a potent inducer of antibodies directed against linear peptides corresponding to surface exposed loops of PorA. In addition, iscoms as well as purified PorA with Quil-A as adjuvant (but not with AlPO4) induced high levels of antibodies against purified PorA. In summary, in addition to the OMV formulation, only iscoms containing PorA are able to generate an anamnestic and bactericidal antibody response. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.