Fast synaptic fatigue in shibire mutants reveals a rapid requirement for dynamin in synaptic vesicle membrane trafficking
Fast synaptic fatigue in shibire mutants reveals a rapid requirement for dynamin in synaptic vesicle membrane trafficking
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DOI：
10.1038/78753
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发表时间：
2000-09-01
影响因子：
25
通讯作者：
Ordway, RW
中科院分区：
文献类型：
--
作者：
Kawasaki, F;Hazen, M;Ordway, RW
GTdR发动蛋白参与许多细胞类型中的内吞作用1，如果蝇发动蛋白基因中的温度敏感性麻痹突变shibire（shi）首次揭示的，其破坏突触囊泡内吞作用并耗尽囊泡的突触末端2，3，4，5，6。在这里，我们报告，shi突触表现出快速突触疲劳表型内20毫秒的重复刺激，这不能解释的囊泡耗尽，我们证实了电子显微镜。这些结果表明，除了其良好的特点在突触囊泡回收的作用，发动蛋白可能需要短期维护的突触囊泡容易释放池。
The GTPase dynamin is involved in endocytosis in many cell types 1, as first revealed by temperature-sensitive paralytic mutations in the Drosophila dynamin gene, shibire (shi), which disrupt synaptic vesicle endocytosis and deplete synaptic terminals of vesicles 2, 3, 4, 5, 6. Here we report that shi synapses exhibit a fast synaptic fatigue phenotype within 20 ms of repetitive stimulation, which cannot be explained by vesicle depletion, as we confirmed by electron microscopy. These results suggest that, in addition to its well-characterized role in synaptic vesicle recycling, dynamin may be required for short-term maintenance of the readily releasable pool of synaptic vesicles.