Intracellular Zn2+ signaling in the dentate gyrus is required for object recognition memory
Intracellular Zn2+ signaling in the dentate gyrus is required for object recognition memory
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齿状回细胞内 Zn2 信号传导是物体识别记忆所必需的
DOI：
10.1002/hipo.22322
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发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
Masaki Ando
中科院分区：
文献类型：
--
作者：
Atsushi Takeda;Haruna Tamano;Taisuke Ogawa;Shunsuke Takada;Masatoshi Nakamura;Hiroaki Fujii;Masaki Ando
研究了穿孔通路-齿状颗粒细胞突触在认知行为中的作用，重点研究了齿状回中突触锌离子的信号转导。当细胞外液中锌离子浓度降低时，目标再认记忆能力受到一过性损害。为探讨细胞内锌离子信号的丢失和/或阻断的作用，将细胞内锌离子螯合剂锌(锌)-2DA(100pmol，0.1 mM/1µL)局部注射到大鼠齿状回分子层内。在训练后1h，注射ZnAF-2DA仅能螯合齿状回细胞内的锌离子信号，但不影响齿状回分子层的细胞内钙信号。在活体齿状回，局部灌流0.1 mM的ZnAF-2DA对记录区域(齿状回颗粒细胞层)的长时程增强(LTP)有影响，但不影响细胞外锌离子的1-10 mM CaEDTA，提示阻断齿状回细胞内的锌离子信号对齿状回的LTP有影响。本研究表明，齿状回细胞内的锌离子信号是物体识别记忆所必需的，可能是通过齿状回LTP的表达。©2014 Wiley期刊，Inc.
The role of perforant pathway‐dentate granule cell synapses in cognitive behavior was examined focusing on synaptic Zn2+signaling in the dentate gyrus. Object recognition memory was transiently impaired when extracellular Zn2+levels were decreased by injection of clioquinol andN,N,N′,N′‐tetrakis‐(2‐pyridylmethyl) ethylendediamine. To pursue the effect of the loss and/or blockade of Zn2+signaling in dentate granule cells, ZnAF‐2DA (100 pmol, 0.1 mM/1 µl), an intracellular Zn2+chelator, was locally injected into the dentate molecular layer of rats. ZnAF‐2DA injection, which was estimated to chelate intracellular Zn2+signaling only in the dentate gyrus, affected object recognition memory 1 h after training without affecting intracellular Ca2+signaling in the dentate molecular layer.In vivodentate gyrus long‐term potentiation (LTP) was affected under the local perfusion of the recording region (the dentate granule cell layer) with 0.1 mM ZnAF‐2DA, but not with 1–10 mM CaEDTA, an extracellular Zn2+chelator, suggesting that the blockade of intracellular Zn2+signaling in dentate granule cells affects dentate gyrus LTP. The present study demonstrates that intracellular Zn2+signaling in the dentate gyrus is required for object recognition memory, probably via dentate gyrus LTP expression. © 2014 Wiley Periodicals, Inc.