Reduced expression of glycolate oxidase leads to enhanced disease resistance in rice.
Reduced expression of glycolate oxidase leads to enhanced disease resistance in rice.
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作者：
Chern M;Bai W;Chen X;Canlas PE;Ronald PC
乙醇酸氧化酶（GLO）是光呼吸过程中的关键酶，催化乙醇酸氧化为乙醛酸。拟南芥GLO是非宿主防御反应所必需的假单胞菌和烟草Pto/AvrPto介导的防御反应。我们先前描述了鉴定与谷氧还蛋白蛋白相互作用的水稻GLO 1，谷氧还蛋白又与TGA转录因子相互作用。TGA转录因子参与NPR 1/NH 1介导的防御信号传导，这对植物系统获得抗性至关重要。在这里，我们证明，减少水稻GLO 1的表达，导致增强的抗黄单胞菌黄单胞菌（Xoo）。GLO 1的组成性沉默导致程序性细胞死亡，导致病变模拟表型和致死性或降低的植物生长和发育，与以前的报道一致。采用地塞米松-GVG（Gal 4 DNA结合结构域-VP 16活化结构域-糖皮质激素受体融合）诱导系统诱导的GLO 1沉默消除了这些有害作用。GLO 1的沉默导致对Xoo的抗性增强，防御调节因子NH 1、NH 3和WRKY 45的表达增加，以及PR 1表达的激活。
Glycolate oxidase (GLO) is a key enzyme in photorespiration, catalyzing the oxidation of glycolate to glyoxylate. Arabidopsis GLO is required for nonhost defense responses to Pseudomonas syringae and for tobacco Pto/AvrPto-mediated defense responses. We previously described identification of rice GLO1 that interacts with a glutaredoxin protein, which in turn interacts with TGA transcription factors. TGA transcription factors are well known to participate in NPR1/NH1-mediated defense signaling, which is crucial to systemic acquired resistance in plants. Here we demonstrate that reduction of rice GLO1 expression leads to enhanced resistance to Xanthomonas oryzae pv oryzae (Xoo). Constitutive silencing of GLO1 leads to programmed cell death, resulting in a lesion-mimic phenotype and lethality or reduced plant growth and development, consistent with previous reports. Inducible silencing of GLO1, employing a dexamethasone-GVG (Gal4 DNA binding domain-VP16 activation domain-glucocorticoid receptor fusion) inducible system, alleviates these detrimental effects. Silencing of GLO1 results in enhanced resistance to Xoo, increased expression of defense regulators NH1, NH3, and WRKY45, and activation of PR1 expression.
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影响因子：
4.2
作者：
Noctor, G;Veljovic-Jovanovic, S;Foyer, CH
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