Characterization of orexins (hypocretins) and melanin-concentrating hormone in genetically obese mice
Characterization of orexins (hypocretins) and melanin-concentrating hormone in genetically obese mice
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DOI：
10.1016/s0167-0115(01)00345-7
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发表时间：
2002-03-15
影响因子：
--
通讯作者：
Matsukura, S
中科院分区：
文献类型：
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作者：
Mondal, MS;Nakazato, M;Matsukura, S
食欲素（hypocretins）和黑色素浓集激素（MCH）是定位于下丘脑外侧区的神经肽，是能量稳态的潜在调节剂。用高灵敏度放射免疫分析法测定了肥胖ob/ob和db/db小鼠及其对照C57 BL/6 J和C57 BL/KSJ小鼠外侧下丘脑（LH）中食欲素和MCH的含量。ob/ob小鼠下丘脑外侧区食欲素含量显著增加，db/db小鼠下丘脑外侧区食欲素含量显著降低。下丘脑外侧区主要的内源性增食欲素分子是成熟的增食欲素-A和-B肽。相反，与对照组小鼠相比，两种肥胖小鼠外侧下丘脑中的MCH含量增加。下丘脑外侧区MCH含量比增食欲素含量高2 ~ 5倍。这些结果提示，在遗传性肥胖小鼠中，食欲素和MCH的调节机制可能不同。(C)2002 Elsevier Science B. V.保留所有权利。
Orexins (hypocretins) and the melanin-concentrating hormone (MCH) are neuropeptides localized to the lateral hypothalamic area and are potential regulators of energy homeostasis. Using highly sensitive radioimmunoassay for orexins and MCH, we determined their contents in the lateral hypothalamus (LH) of genetically obese ob/ob and db/db mice and their controls, C57BL/6J and C57BL/KSJ. The orexin contents in the lateral hypothalamus significantly increased in the ob/ob mice, whereas the orexin contents significantly decreased in the db/db mice. Mature orexin-A and -B peptides were the major endogenous orexin molecules in the lateral hypothalamus. Conversely, the MCH contents in the lateral hypothalamus of both obese mice increased compared to the control mice. MCH contents in the lateral hypothalamus were two- to five-Cold higher than that of orexin contents. These results suggest that the regulatory mechanism of orexin and MCH may be different in the genetically obese mice. (C) 2002 Elsevier Science B.V. All rights reserved.