DIFFERENCES IN INVOLUCRIN IMMUNOLABELING WITHIN CORNIFIED CELL ENVELOPES IN NORMAL AND PSORIATIC EPIDERMIS
DIFFERENCES IN INVOLUCRIN IMMUNOLABELING WITHIN CORNIFIED CELL ENVELOPES IN NORMAL AND PSORIATIC EPIDERMIS
复制标题
DOI：
10.1111/1523-1747.ep12665870
复制
发表时间：
1995-03-01
影响因子：
6.5
通讯作者：
IIZUKA, H
中科院分区：
文献类型：
--
作者：
ISHIDAYAMAMOTO, A;IIZUKA, H
表皮角质形成细胞在分化后期在质膜下方形成角化细胞包膜 (CE)，该 CE 通过几种前体蛋白（包括外皮蛋白）的交联来稳定。在牛皮癣中，已知前体蛋白的表达模式被扰乱；外皮蛋白表达增加，兜甲蛋白表达减少，然而，这些变化之前尚未进行超微结构评估。在本研究中，我们结合传统的透射电子显微镜进行了光学和电子显微镜免疫组织化学分析，以评估正常和银屑病CE中外皮蛋白参与的性质。在正常表皮中，从最深的角化细胞或（如果存在）从移行细胞中观察到CE，随着成熟，厚度增加并改变电子密度。在银屑病表皮中，CE形成较早开始，角化层下方的一到几个细胞。银屑病CE通常较薄，并显示出恒定的高电子密度，免疫电镜显示正常CE仅在非常早期阶段为外皮蛋白阳性，而银屑病CE显示持续的外皮蛋白免疫反应性。这些结果表明，在正常皮肤中，外皮蛋白仅在其组装的早期阶段才是CE的主要成分，相反，CE形成似乎在银屑病皮肤中过早开始，其中外皮蛋白在成熟过程中仍然是CE的主要成分。
Epidermal keratinocytes form a cornified cell envelope (CE) beneath the plasma membrane during the late stages of differentiation, This CE is stabilized by cross linking of several precursor proteins, including involucrin, In psoriasis, the expression pattern of the precursor proteins is known to be deranged; involucrin expression is increased and loricrin expression is decreased, However, these changes have not been previously evaluated ultrastructurally. In the present study, we performed light and electron microscopic immunohistochemistry in conjunction with conventional transmission electron microscopy to assess the nature of involucrin involvement in normal and psoriatic CE. In normal epidermis, CEs were observed from the deepest cornified cells or, when present, from the transitional cells, increasing in thickness and changing electron densities with maturation, In psoriatic epidermis, CE formation started earlier, one to several cells below the cornified layer. Psoriatic CEs were generally thinner and showed a constant high electron density, Immunoelectron microscopy revealed that the normal CE was involucrin positive only at a very early stage, whereas psoriatic CE showed persistent involucrin immunoreactivity, These results suggest that in normal skin, involucrin is the major constituent of the CE only in its early stages of assembly, In contrast, CE formation seems to be initiated prematurely in psoriatic skin, where involucrin remains the major constituent of the CE during maturation.