Genetic control of root architectural traits in KDML105 chromosome segment substitution lines under well-watered and drought stress conditions
Genetic control of root architectural traits in KDML105 chromosome segment substitution lines under well-watered and drought stress conditions
复制标题
DOI：
10.1080/1343943x.2021.1883990
复制
发表时间：
2021-02
影响因子：
2.5
通讯作者：
Mathurada Ruangsiri;P. Vejchasarn;P. Saengwilai;J. Lynch;M. Bennett;K. Brown;C. Chutteang;R. Boonruang
中科院分区：
文献类型：
--
作者：
Mathurada Ruangsiri;P. Vejchasarn;P. Saengwilai;J. Lynch;M. Bennett;K. Brown;C. Chutteang;R. Boonruang
摘要干旱是水稻生产的主要限制因素，根构型性状是干旱胁迫下水稻生产力改良的重要育种指标。一组染色体片段置换系（KDML 105-CSSLs）和KDML 105在泰国的两个地点（水稻基因发现（Rice Gene Discovery，RGD）和乌汶Ratchatani水稻研究中心（Ubon Rice Research Center，URRC））在湿润季节在充分浇水（WW）和干旱胁迫（DS）处理下生长。RGD的特点是具有重粘土类型，而URRC的土壤具有高比例的沙子和特点是不育。根构型性状在两个网站的人口变化，并表现出可塑性，在响应干旱的位置受水分状况的相互作用。侧根密度增加了77%，干旱在RGD，但下降了18%，在URRC。干旱胁迫下，伸长更多的垂直节根的比例增加了21%，在RGD。干旱条件下，根数与分蘖数和生物量呈负相关，而侧根密度与干旱条件下的生物量呈负相关。确定了8个控制分蘖节根数、侧根密度和节根生长角度的QTL。通过注释QTL区域内的基因来鉴定几个候选基因。我们的研究提出了根构型性状的遗传见解，在水稻耐旱育种计划中具有潜在的用途。图形摘要
ABSTRACT Drought is a major constraint in rainfed rice production and root architectural traits are important breeding targets for improving productivity under drought stress. A set of chromosome segment substitution lines (KDML105-CSSLs) and KDML105 were grown in the wet season at two sites (Rice Gene Discovery (RGD) and Ubon Ratchatani Rice Research Center (URRC)) in Thailand under well-watered (WW) and drought stress (DS) treatments. RGD is characterized by having a heavy clay soil type while URRC’s soil has a high percentage of sand and characterized by infertility. Root architecture traits varied within the population at both sites and exhibited plasticity in response to drought as affected by location by water regime interaction. Lateral root density increased by 77% with drought at RGD but decreased by 18% at URRC. The proportion of nodal roots that elongated more vertically increased under drought stress by 21%, at RGD. Root number per tiller was negatively associated with tiller number and biomass at RGD under drought, while lateral root density was negatively associated with biomass under drought at URRC. Eight QTL were identified for the number of nodal roots per tiller, lateral root density, and nodal root growth angle. Several candidate genes were identified by annotating the genes within the QTL regions. Our study presented genetic insights into root architectural traits with potential use in rice breeding programs for drought tolerance. Graphical abstract