A Biosensor Based on Zucchini (Cucurbita Pepo L.) Homogenate as a Biorecognition Layer for Ascorbic Acid Determination
A Biosensor Based on Zucchini (Cucurbita Pepo L.) Homogenate as a Biorecognition Layer for Ascorbic Acid Determination
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DOI：
10.3109/10731191003785588
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发表时间：
2010-07
期刊：
影响因子：
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通讯作者：
M. Sezgintürk;H. Koca;Yavuz Selim Özben;E. Dinçkaya
中科院分区：
文献类型：
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作者：
M. Sezgintürk;H. Koca;Yavuz Selim Özben;E. Dinçkaya
摘要以西葫芦组织匀浆为基质，研制了一种电流型生物传感器。西葫芦组织匀浆用戊二醛与明胶交联，并固定在预处理的聚四氟乙烯膜上。西葫芦组织中含有抗坏血酸氧化酶，这种酶在溶解氧的存在下催化抗坏血酸的氧化。测量原理基于溶解氧水平降低的测定。通过标准曲线进行测定，所述标准曲线通过测量与抗坏血酸浓度相关的氧水平的降低而获得。对该生物传感器进行了优化和表征研究。首先，对西葫芦组织匀浆、明胶和戊二醛的用量进行了优化。对缓冲体系、pH值、缓冲液浓度、温度等实验参数进行了优化。研究了该传感器的热稳定性、储存稳定性和重复性。在5×10−6 M至1.2×10−3 M抗坏血酸范围内观察到线性响应。最后，将该生物传感器与分光光度法（Tillman试剂）进行了比较，并对植物和药物样品进行了分析。
Abstract An amperometric biosensor based on zucchini (Cucurbita pepo) tissue homogenate is presented. The zucchini tissue homogenate was crosslinked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. The zucchini tissue contained the enzyme ascorbate oxidase and this enzyme catalyzed the oxidation of ascorbic acid in the presence of dissolved oxygen. The principle of the measurements was based on the determination of the decrease in the dissolved oxygen level. Determinations were carried out by standard curves, which were obtained by the measurement of the decrease in the oxygen level related to ascorbic acid concentration. Optimization and characterization studies of the biosensor were carried out in detail. First of all, the amounts of zucchini tissue homogenate, gelatin, and glutaraldehyde percentage were optimized. Experimental parameters such as buffer system, pH, buffer concentration, and temperature were also optimized carefully. Thermal stability, storage stability, and repeatability of the biosensor were investigated. A linear response was observed from 5×10−6 M to 1.2×10−3 M ascorbic acid. Finally, the results of some plant and drug samples analyzed with the presented biosensor compared with the spectrophotometric method (Tillman reagent) used as a reference.