The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis.
The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis.
复制标题
异二聚体的非常晚期抗原家族是参与粘附和胚胎发生的分子超家族的一部分。
DOI：
--
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
M. Hemler
中科院分区：
文献类型：
--
作者：
Yoshikazu Takada;J. L. Strominger;M. Hemler
极晚期抗原（VLA）蛋白家族包含至少五个相关的异源二聚体，包括纤连蛋白受体结构，以及可能的其他细胞底物粘附受体。使用一系列单克隆抗体-琼脂糖免疫亲和柱从人胎盘（VLA-1、VLA-3和VLA-5）、血小板（VLA-2）和Molt-4细胞（VLA-4）免疫纯化这些细胞表面VLA蛋白。通过凝胶电泳进一步纯化后，测定了五个VLA α亚基中每一个的N-末端氨基酸序列。在前14个位置中，5个VLA α亚基显示出彼此平均42%的同源性，包括保守的氨基酸取代，同源性上升至59%。此外，来自LFA-1、Mac-1（CR-3）和p150、95异二聚体家族、玻连蛋白受体-血小板GPIIb/IIIa家族和在果蝇胚胎发生中重要的位置特异性（PS）抗原的α亚基均显示与单个VLA α序列的平均同源性为31-40%，与VLA α亚基（包括保守取代）的平均同源性为46-52%。两者合计，这些结果表明，VLA蛋白，LFA-1，Mac-1，和p150，95家族，GPIIb/IIIa，玻连蛋白受体家族，和果蝇PS抗原已演变为一个高度保守的超基因家族的受体参与的基本重要的功能，如细胞粘附，迁移和胚胎发生的四个亚组。
The very late antigen (VLA) protein family contains at least five related heterodimers, including a fibronectin receptor structure, and probably other cell substrate adhesion receptors. These cell-surface VLA proteins were immunopurified from human placenta (VLA-1, VLA-3, and VLA-5), platelets (VLA-2), and Molt-4 cells (VLA-4) using a series of monoclonal antibody-Sepharose immunoaffinity columns. After further purification by gel electrophoresis, the N-terminal amino acid sequence for each of the five VLA alpha subunits was determined. In the first 14 positions, the five VLA alpha subunits showed an average of 42% homology to each other, rising to 59% including conservative amino acid substitutions. In addition, the alpha subunits from the LFA-1, Mac-1 (CR-3), and p150,95 family of heterodimers, the vitronectin receptor-platelet GPIIb/IIIa family, and a position-specific (PS) antigen important in Drosophila embryogenesis each showed average homologies of 31-40% to individual VLA alpha sequences and 46-52% homology to VLA alpha subunits including conservative substitutions. Taken together, these results suggest that the VLA proteins, the LFA-1, Mac-1, and p150,95 family, the GPIIb/IIIa, vitronectin receptor family, and the Drosophila PS antigens have evolved as four subgroups in a highly conserved supergene family of receptors involved in fundamentally important functions, such as cell adhesion, migration, and embryogenesis.