Gastrodin alleviates Tourette syndrome via Nrf-2/HO-1/HMGB1/NF-kB pathway
Gastrodin alleviates Tourette syndrome via Nrf-2/HO-1/HMGB1/NF-kB pathway
复制标题
天麻素通过 Nrf-2/HO-1/HMGB1/NF-kB 通路缓解抽动秽语综合征
DOI：
10.1002/jbt.22389
复制
发表时间：
2019-08-30
影响因子：
3.6
通讯作者：
Huang, Yaruo
中科院分区：
文献类型：
--
作者：
Long, Hongyan;Ruan, Jie;Huang, Yaruo
目的：研究天麻素（GA）对抽动秽语综合征（TS）大鼠纹状体炎症及氧化应激的影响。用3，3 '-亚氨基二丙腈诱导大鼠TS模型。行为测试采用刻板印象实验。采用高效液相色谱法测定了纹状体中氨基酸递质谷氨酸（Glu）和γ-氨基丁酸（GABA）的浓度。用试剂盒检测血清和纹状体超氧化物歧化酶（SOD）和丙二醛（MDA）。酶联免疫吸附法检测血清和纹状体细胞因子。Western blot检测纹状体核红细胞因子2相关因子2（Nrf-2）/血红素加氧酶-1（HO-1）/高迁移率族蛋白B1（HMGB 1）/核因子-kB（NF-kB）通路相关蛋白。免疫组化法检测纹状体Nrf-2和P-NF-kBp 65的表达。与对照组相比，模型组大鼠刻板印象评分明显增加，纹状体Glu和GABA含量明显升高。GA显著降低刻板印象得分，降低Glu和GABA含量。与对照组相比，血清和纹状体中SOD含量降低，MDA含量升高，GA可明显恢复上述变化。GA显著调节Nrf-2/HO-1/HMGB 1/NF-kB通路相关蛋白的变化，与免疫组化变化一致。GA可能通过调节纹状体Nrf-2/HO-1/HMGB 1/NF-kB通路蛋白的变化，对TS大鼠纹状体起到保护作用。
The aim is to study the effects of gastrodin (GA) on striatal inflammation and oxidative stress in rats with Tourette syndrome (TS). The rat model of TS was induced by 3,3 '-iminodipropionitrile. Behavioral tests were carried out by stereotype experiment. The concentrations of amino acid transmitters glutamic acid (Glu) and gamma-aminobutyric acid (GABA) in striatum were determined by high-performance liquid chromatography. Superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and striatum were detected by commercial kits. Cytokines in serum and striatum were detected by enzyme-linked immunosorbent assay kits. Western blot analysis was used to detect striatum nuclear erythroid factor 2-related factor 2 (Nrf-2)/heme oxygenase-1 (HO-1)/high mobility group box 1 protein (HMGB1)/nuclear factor-kB (NF-kB) pathway-related proteins. The expressions of Nrf-2 and P-NF-kBp65 in striatum were detected by immunohistochemistry. Compared with the control group, the stereotype scores of rats in the model group significantly increased, and the contents of Glu and GABA in striatum obviously increased. GA significantly reduced the stereotype scores and decreased the contents of Glu and GABA. The levels of SOD in serum and striatum were decreased and the content of MDA in serum and striatum were increased compared with the control group, while GA significantly restored the changes. GA significantly adjusted Nrf-2/HO-1/HMGB1/NF-kB pathway-related proteins changes consistent with immunohistochemical changes. GA may protect striatum of rats with TS by regulating Nrf-2/HO-1/HMGB1/NF-kB pathway protein changes in striatum.